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Literature summary for 5.5.1.20 extracted from

  • Katayama, K.; Kobayashi, T.; Oikawa, H.; Honma, M.; Ichihara, A.
    Enzymatic activity and partial purification of solanapyrone synthase: First enzyme catalyzing Diels-Alder reaction (1998), Biochim. Biophys. Acta, 1384, 387-395.
    View publication on PubMed
Search for more literature for 5.5.1.20

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000 60000 gel filtration Alternaria solani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
prosolanapyrone III Alternaria solani the enzyme is involved in solanapyrone biosynthesis (-)-solanapyrone A
-
 ?

Organism

Organism UniProt Comment Textmining
Alternaria solani D7UQ40
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Alternaria solani

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Alternaria solani
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
prosolanapyrone III the enzyme is involved in solanapyrone biosynthesis Alternaria solani (-)-solanapyrone A
-
 ?
prosolanapyrone III the bifunctional enzyme catalyses the oxidation of prosolanapyrone II and the subsequent cycloaddition of the product prosolanapyrone III to (-)-solanapyrone A Alternaria solani (-)-solanapyrone A in an enzyme-catalyzed Diels–Alder reaction the product prosolanapyrone III is isomerized to solanapyrone A (exo Diels-Alder cyclization, [4+2] cycloaddition). Solanapyrone D (endo Diels-Alder cyclization) is formed by a nonenzymatic Diels-Alder cyclization of prosolanopyrone III in polar solvents. The enzyme coverts prosolanapyrone II into solanapyrone A, solanapyrone D and prosolanapyrone III in the ratio 7:1:1 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Alternaria solani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Alternaria solani

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5.0: about 55% of maximal activity, pH 9.0: about 55% of maximal activity Alternaria solani

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.7
-
 irreversible inactivation below Alternaria solani

pI Value

Organism Comment pI Value Maximum pI Value
Alternaria solani isoelectric focusing
-
 4.25