Literature summary for 5.5.1.2 extracted from

Yang, J.; Wang, Y.; Woolridge, E.M.; Arora, V.; Petsko, G.A.; Kozarich, J.W.; Ringe, D.
Crystal structure of 3-carboxy-cis,cis-muconate lactonizing enzyme from Pseudomonas putida, a fumarase class II type cycloisomerase: Enzyme Evolution in Parallel Pathways (2004), Biochemistry, 43, 10424-10434.

Search for more literature for 5.5.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain N4830	Pseudomonas putida

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion method with 12-18% PEG8000, 5 mM dithiothreitol, and 40 mM sodium phosphate (pH 7.3)	Pseudomonas putida
hanging-drop vapor-diffusion method, 2.6 A resolution, symmetric of hexagonal space group P6(5) with unit cell parameters: a = b = 231.52 A, c = 78.46 A, alpha = beta = 90°, gamma = 120°. There are four PpCMLE molecules, forming a homotetramer, in the asymmetric unit	Pseudomonas putida

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	requires no metal cofactor for catalytic activity	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas putida
DEAE-Sepharcel column chromatography	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-carboxy-cis,cis-muconate	-	Pseudomonas putida	4-carboxy-(S)-muconolactone	-	r
additional information	adenylosuccinate is not a substrate	Pseudomonas putida	?	-	?

Subunits

Subunits	Comment	Organism
homotetramer	x-ray crystallography	Pseudomonas putida
tetramer	-	Pseudomonas putida

Synonyms

Synonyms	Comment	Organism
3-Carboxy-cis,cis-muconate lactonizing enzyme	-	Pseudomonas putida
3-Carboxy-cis,cis-muconate lactonizing enzyme	belongs to the fumarase class II superfamily	Pseudomonas putida
CMLE	-	Pseudomonas putida
PpCMLE	-	Pseudomonas putida

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Release 2023.1 (January 2023)