Any feedback?
Literature summary for 5.5.1.19 extracted from
- Overexpression and characterization of lycopene cyclase (CrtY) from marine bacterium Paracoccus haeundaensis (2013), J. Microbiol. Biotechnol., 23, 144-148.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene crtY, DNA and amino acid sequence determination and analysis, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) codon plus, 25°C is the optimum growth temperature for obtaining soluble lycopene cyclase in an active form | Paracoccus haeundaensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
lycopene | pH 6.5, 30°C, recombinant enzyme | Paracoccus haeundaensis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000, recombinant enzyme, SDS-PAGE | Paracoccus haeundaensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lycopene | Paracoccus haeundaensis | - |
beta-carotene | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paracoccus haeundaensis | Q24K62 | gene crtY | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography to homogeneity | Paracoccus haeundaensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
recombinant enzyme expression yielding 103 ng beta-carotene/ml supernatant/h | Paracoccus haeundaensis |
| 0.356 | - |
purified recombinant enzyme, pH 6.5, 30°C | Paracoccus haeundaensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lycopene | - |
Paracoccus haeundaensis | beta-carotene | - |
? | |
| lycopene | lycopene cyclase converts lycopene to beta-carotene by catalyzing the formation of two beta-rings at each end of the linear carotene structure. Two-step reaction in which both sides of of the lycopene molecule are cyclized into beta-carotene rings via the monocyclic gamma-carotene as an intermediate | Paracoccus haeundaensis | beta-carotene | - |
? | |
| additional information | substrate specificity, recombinant enzyme, overview | Paracoccus haeundaensis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, recombinant enzyme, SDS-PAGE | Paracoccus haeundaensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CrtY | - |
Paracoccus haeundaensis |
| lycopene cyclase | - |
Paracoccus haeundaensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
recombinant enzyme | Paracoccus haeundaensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
recombinant enzyme | Paracoccus haeundaensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | required, Km value is 2 mM | Paracoccus haeundaensis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
