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Literature summary for 5.5.1.17 extracted from

  • Köllner, T.G.; Schnee, C.; Li, S.; Svatos, A.; Schneider, B.; Gershenzon, J.; Degenhardt, J.
    Protonation of a neutral (S)-beta-bisabolene intermediate is involved in (S)-beta-macrocarpene formation by the maize sesquiterpene synthases TPS6 and TPS11 (2008), J. Biol. Chem., 283, 20779-20788.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.5.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Zea mays
overexpression in Escherichia coli Zea mays

Protein Variants

Protein Variants Comment Organism
D526N inactive Zea mays
D526N mutation inactivates the enzyme completely Zea mays
Y522F the mutation strongly reduces the rate of reprotonation of (S)-beta-bisabolene Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
 geranyl diphosphate recombinant enzyme, in the presence of 0.05 mM Mn2+, pH and temperature not specified in the publication Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Zea mays 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ TPS6 requires a divalent metal ion, with Mg2+ and Mn2+ being effective, optimum activity with 5 mM Mg2+ and 5 mM Mn2+. Although the Km for Mn2+ is significantly lower than for Mg2+, the enzyme is more likely to operate with a Mg2+ cofactor in planta, because the concentration of Mg2+ in plant cells is about 2 orders of magnitudes higher than Mn2+. In the presence of Mn2+, the product spectrum of TPS6 is shifted toward an increased production of (S)-beta-bisabolene and a decreased production of (S)-beta-macrocarpene. KM-value for Mg2+ measured with 0.01 mM farnesyl diphosphate: 0.131 mM Zea mays
Mg2+ the enzyme is mostly stimulated by 10 mM Mg2+ Zea mays
Mn2+ TPS6 requires a divalent metal ion, with Mg2+ and Mn2+ being effective, optimum activity with 5 mM Mg2+ and 5 mM Mn2+. Although the Km for Mn2+ is significantly lower than for Mg2+, the enzyme is more likely to operate with a Mg2+ cofactor in planta, because the concentration of Mg2+ in plant cells is about 2 orders of magnitudes higher than Mn2+. In the presence of Mn2+, the product spectrum of TPS6 is shifted toward an increased production of (S)-beta-bisabolene and a decreased production of (S)-beta-macrocarpene. KM-value for Mn2+ measured with 0.01 mM farnesyl diphosphate: 0.0234 mM Zea mays
Mn2+ the enzyme is stimulated by 0.25 mM Mn2+. In the presence of Mn2+, there is increased formation of (S)-beta-bisabolene Zea mays
additional information no stimulation of activity is observed in the presence of Ca2+, Cd2+, Co2+, Cu2+, Ni2+, and Zn2+ (0.25 and 5 mM each) Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-beta-bisabolene Zea mays the enzyme may be involved in plant defense (S)-beta-macrocarpene
-
 ?
(S)-beta-bisabolene Zea mays the enzyme may be involved in plant defense (S)-beta-macrocarpene i.e. (1'S)-4',5,5-trimethyl-1,1'-bi(cyclohexane)-1,3'-diene. Product is identified by GC-MS ?

Organism

Organism UniProt Comment Textmining
Zea mays Q1EG72 variety B73
-
Zea mays Q1EG72 L. variety B73
-
Zea mays Q5GJ60 variety B73
-
Zea mays Q5GJ60 L. variety B73
-

Purification (Commentary)

Purification (Comment) Organism
partial Zea mays
partial purification Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Zea mays
-
root
-
 Zea mays
-
root the enzyme is predominantly active in the roots Zea mays
-
seedling
-
 Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-beta-bisabolene
-
 Zea mays (S)-beta-macrocarpene i.e. (1'S)-4',5,5-trimethyl-1,1'-bi(cyclohexane)-1,3'-diene. Product is identified by GC-MS ?
(S)-beta-bisabolene the enzyme may be involved in plant defense Zea mays (S)-beta-macrocarpene
-
 ?
(S)-beta-bisabolene the enzyme may be involved in plant defense Zea mays (S)-beta-macrocarpene i.e. (1'S)-4',5,5-trimethyl-1,1'-bi(cyclohexane)-1,3'-diene. Product is identified by GC-MS ?
(S)-beta-bisabolene the synthesis of (S)-macrocarpene from (2E,6E)-farnesyl diphosphate in two steps. The first step is the cyclization to (S)-beta-. The second step is the isomerization to (S)-beta-macrocarpene ((S)-beta-macrocarpene synthase) Zea mays (S)-beta-macrocarpene i.e. (1'S)-4',5,5-trimethyl-1,1'-bi(cyclohexane)-1,3'-diene. Product is identified by GC-MS ?
(S)-beta-bisabolene (S)-beta-macrocarpene formation by TPS11 requires a protonation of the (S)-beta-bisabolene intermediate Zea mays (S)-beta-macrocarpene
-
 ?
geranyl diphosphate
-
 Zea mays (S)-beta-bisabolene + (S)-beta-macrocarpene in the presence of geranylgeranyl diphosphate, TPS11 also catalyzes the formation of beta-myrcene and linalool, along with minor amounts of limonene, alpha-thujene, sabinene, and alpha-terpinolene ?
additional information no enzymatic activity is observed in the presence of geranylgeranyl diphosphate Zea mays ?
-
 ?

Synonyms

Synonyms Comment Organism
TPS11
-
 Zea mays
TPS6
-
 Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 in presence of 5 mM Mg2+ Zea mays
7
-
 forming beta-macrocarpene Zea mays
8.5
-
 forming beta-bisabolene Zea mays

pH Range

pH Minimum pH Maximum Comment Organism
6.2 8.6 half-maximal activity at pH 6.2 and at pH 8.6, in presence of 5 mM Mg2+. Within a pH range from 5.0 to 8.0, the major product is (S)-beta-macrocarpene, but higher pH values favor the formation of (S)-beta-bisabolene Zea mays

Expression

Organism Comment Expression
Zea mays after leaf damage by Spodoptera littoralis, the transcript level of TSP11 is elevated up
Zea mays after leaf damage by Spodoptera littoralis, the transcript level of TSP6 is elevated up

General Information

General Information Comment Organism
physiological function the enzyme may be involved in plant defense Zea mays