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Literature summary for 5.5.1.12 extracted from

  • Hall, D.; Zerbe, P.; Jancsik, S.; Quesada, A.; Dullat, H.; Madilao, L.; Yuen, M.; Bohlmann, J.
    Evolution of conifer diterpene synthases: diterpene resin acid biosynthesis in lodgepole pine and jack pine involves monofunctional and bifunctional diterpene synthases (2013), Plant Physiol., 161, 600-616.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged enzyme in Escherichia coli Pinus banksiana
expression of His-tagged enzyme in Escherichia coli Pinus contorta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate Pinus banksiana
-
(+)-copalyl diphosphate
-
?
geranylgeranyl diphosphate Pinus contorta
-
(+)-copalyl diphosphate
-
?
additional information Pinus banksiana gymnosperm diterpene synthases of diterpene resin acid biosynthesis are bifunctional enzymes that catalyze the initial bicyclization of geranylgeranyl diphosphate followed by rearrangement of a (+)-copalyl diphosphate intermediate at two discrete class II and class I active sites. The bifunctional jack pine and lodgepole pine LAS enzymes demonstrated that the class I active sites of monofunctional proteins can use the (+)-copalyl diphosphate intermediate produced by the class II active site of the bifunctional enzymes, overview ?
-
?
additional information Pinus contorta gymnosperm diterpene synthases of diterpene resin acid biosynthesis are bifunctional enzymes that catalyze the initial bicyclization of geranylgeranyl diphosphate followed by rearrangement of a (+)-copalyl diphosphate intermediate at two discrete class II and class I active sites. The bifunctional jack pine and lodgepole pine LAS enzymes demonstrated that the class I active sites of monofunctional proteins can use the (+)-copalyl diphosphate intermediate produced by the class II active site of the bifunctional enzymes, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Pinus banksiana M4HXU6 LAS1
-
Pinus contorta M4HXW7 class II diTPS
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Pinus banksiana
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Pinus contorta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate
-
Pinus banksiana (+)-copalyl diphosphate
-
?
geranylgeranyl diphosphate
-
Pinus contorta (+)-copalyl diphosphate
-
?
additional information gymnosperm diterpene synthases of diterpene resin acid biosynthesis are bifunctional enzymes that catalyze the initial bicyclization of geranylgeranyl diphosphate followed by rearrangement of a (+)-copalyl diphosphate intermediate at two discrete class II and class I active sites. The bifunctional jack pine and lodgepole pine LAS enzymes demonstrated that the class I active sites of monofunctional proteins can use the (+)-copalyl diphosphate intermediate produced by the class II active site of the bifunctional enzymes, overview Pinus banksiana ?
-
?
additional information gymnosperm diterpene synthases of diterpene resin acid biosynthesis are bifunctional enzymes that catalyze the initial bicyclization of geranylgeranyl diphosphate followed by rearrangement of a (+)-copalyl diphosphate intermediate at two discrete class II and class I active sites. The bifunctional jack pine and lodgepole pine LAS enzymes demonstrated that the class I active sites of monofunctional proteins can use the (+)-copalyl diphosphate intermediate produced by the class II active site of the bifunctional enzymes, overview Pinus contorta ?
-
?

Synonyms

Synonyms Comment Organism
bifunctional LAS-type diTPS
-
Pinus banksiana
bifunctional LAS-type diTPS
-
Pinus contorta
class II diterpene synthase
-
Pinus banksiana
class II diterpene synthase
-
Pinus contorta
class II diTPS
-
Pinus banksiana
class II diTPS
-
Pinus contorta
ISO
-
Pinus banksiana
ISO
-
Pinus contorta
isopimaradiene synthase-type diTPS
-
Pinus banksiana
isopimaradiene synthase-type diTPS
-
Pinus contorta
LAS
-
Pinus banksiana
LAS
-
Pinus contorta
LAS1
-
Pinus banksiana
levopimaradiene/abietadiene synthase-type diTPS
-
Pinus banksiana
levopimaradiene/abietadiene synthase-type diTPS
-
Pinus contorta

General Information

General Information Comment Organism
evolution the enzyme belongs to the class II diterpene synthases, phylogenetic tree, diterpene biosynthesis in gymnosperms, overview Pinus banksiana
evolution the enzyme belongs to the class II diterpene synthases, phylogenetic tree, diterpene biosynthesis in gymnosperms, overview Pinus contorta
metabolism bifunctional class I/II PcLAS1 and PbLAS1 enzymes can supply (+)-copalyl diphosphate for the Monofunctional class I enzymes Pinus banksiana