Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli | Pinus banksiana |
expression of His-tagged enzyme in Escherichia coli | Pinus contorta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | Pinus banksiana | - |
(+)-copalyl diphosphate | - |
? | |
geranylgeranyl diphosphate | Pinus contorta | - |
(+)-copalyl diphosphate | - |
? | |
additional information | Pinus banksiana | gymnosperm diterpene synthases of diterpene resin acid biosynthesis are bifunctional enzymes that catalyze the initial bicyclization of geranylgeranyl diphosphate followed by rearrangement of a (+)-copalyl diphosphate intermediate at two discrete class II and class I active sites. The bifunctional jack pine and lodgepole pine LAS enzymes demonstrated that the class I active sites of monofunctional proteins can use the (+)-copalyl diphosphate intermediate produced by the class II active site of the bifunctional enzymes, overview | ? | - |
? | |
additional information | Pinus contorta | gymnosperm diterpene synthases of diterpene resin acid biosynthesis are bifunctional enzymes that catalyze the initial bicyclization of geranylgeranyl diphosphate followed by rearrangement of a (+)-copalyl diphosphate intermediate at two discrete class II and class I active sites. The bifunctional jack pine and lodgepole pine LAS enzymes demonstrated that the class I active sites of monofunctional proteins can use the (+)-copalyl diphosphate intermediate produced by the class II active site of the bifunctional enzymes, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pinus banksiana | M4HXU6 | LAS1 | - |
Pinus contorta | M4HXW7 | class II diTPS | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Pinus banksiana |
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Pinus contorta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | - |
Pinus banksiana | (+)-copalyl diphosphate | - |
? | |
geranylgeranyl diphosphate | - |
Pinus contorta | (+)-copalyl diphosphate | - |
? | |
additional information | gymnosperm diterpene synthases of diterpene resin acid biosynthesis are bifunctional enzymes that catalyze the initial bicyclization of geranylgeranyl diphosphate followed by rearrangement of a (+)-copalyl diphosphate intermediate at two discrete class II and class I active sites. The bifunctional jack pine and lodgepole pine LAS enzymes demonstrated that the class I active sites of monofunctional proteins can use the (+)-copalyl diphosphate intermediate produced by the class II active site of the bifunctional enzymes, overview | Pinus banksiana | ? | - |
? | |
additional information | gymnosperm diterpene synthases of diterpene resin acid biosynthesis are bifunctional enzymes that catalyze the initial bicyclization of geranylgeranyl diphosphate followed by rearrangement of a (+)-copalyl diphosphate intermediate at two discrete class II and class I active sites. The bifunctional jack pine and lodgepole pine LAS enzymes demonstrated that the class I active sites of monofunctional proteins can use the (+)-copalyl diphosphate intermediate produced by the class II active site of the bifunctional enzymes, overview | Pinus contorta | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bifunctional LAS-type diTPS | - |
Pinus banksiana |
bifunctional LAS-type diTPS | - |
Pinus contorta |
class II diterpene synthase | - |
Pinus banksiana |
class II diterpene synthase | - |
Pinus contorta |
class II diTPS | - |
Pinus banksiana |
class II diTPS | - |
Pinus contorta |
ISO | - |
Pinus banksiana |
ISO | - |
Pinus contorta |
isopimaradiene synthase-type diTPS | - |
Pinus banksiana |
isopimaradiene synthase-type diTPS | - |
Pinus contorta |
LAS | - |
Pinus banksiana |
LAS | - |
Pinus contorta |
LAS1 | - |
Pinus banksiana |
levopimaradiene/abietadiene synthase-type diTPS | - |
Pinus banksiana |
levopimaradiene/abietadiene synthase-type diTPS | - |
Pinus contorta |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the class II diterpene synthases, phylogenetic tree, diterpene biosynthesis in gymnosperms, overview | Pinus banksiana |
evolution | the enzyme belongs to the class II diterpene synthases, phylogenetic tree, diterpene biosynthesis in gymnosperms, overview | Pinus contorta |
metabolism | bifunctional class I/II PcLAS1 and PbLAS1 enzymes can supply (+)-copalyl diphosphate for the Monofunctional class I enzymes | Pinus banksiana |