Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ binds deep within a non-solvent exposed cavity, coordinated by 2 aspartate and 1 glutamic acid residues, a single water molecule and the substrate, in a distorted octahedral form. The carboxylate of cis,cis-muconate binds across the Mg2+ ion in conformations that display mirror-like symmetry in the two different proteins | Mycolicibacterium smegmatis | |
Mg2+ | Mg2+ binds deep within a non-solvent exposed cavity, coordinated by 2 aspartate and 1 glutamic acid residues, a single water molecule and the substrate, in a distorted octahedral form. The carboxylate of cis,cis-muconate binds across the Mg2+ ion in conformations that display mirror-like symmetry in the two different proteins | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Pseudomonas fluorescens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(+)-muconolactone = cis,cis-muconate | reaction mechanism, DFT QM/MM calculations and structure-function relationship, overview | Mycolicibacterium smegmatis | |
(+)-muconolactone = cis,cis-muconate | reaction mechanism, DFT QM/MM calculations and structure-function relationship, overview | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis,cis-hexadienedioate | conversion of cis,cis-muconate to muconolactone, two proximal Lys residues exist in the active sites, which can potentially lead, via a single intermediate form, to the formation of either the syn- or anti-products. The enzyme from Mycobacterium smegmatis forms a syn-product, overview | Mycolicibacterium smegmatis | 2,5-dihydro-5-oxofuran-2-acetate | - |
r | |
cis,cis-hexadienedioate | conversion of cis,cis-muconate to muconolactone, two proximal Lys residues exist in the active sites, which can potentially lead, via a single intermediate form, to the formation of either the syn- or anti-products. The enzyme from Pseudomonas fluorescens forms an anti-product, overview | Pseudomonas fluorescens | 2,5-dihydro-5-oxofuran-2-acetate | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | TIM barrel-like alpha/beta protein fold | Mycolicibacterium smegmatis |
More | TIM barrel-like alpha/beta protein fold | Pseudomonas fluorescens |
Synonyms | Comment | Organism |
---|---|---|
anti-MLE | - |
Mycolicibacterium smegmatis |
cis,cis-muconate lactonizing enzyme | - |
Mycolicibacterium smegmatis |
cis,cis-muconate lactonizing enzyme | - |
Pseudomonas fluorescens |
MLE | - |
Mycolicibacterium smegmatis |
MLE | - |
Pseudomonas fluorescens |
syn-MLE | - |
Pseudomonas fluorescens |