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Literature summary for 5.5.1.1 extracted from
- Evolution of enzymatic activities in the enolase superfamily: Stereochemically distinct mechanisms in two famillies of cis,cis-muconate lactonizing enzymes (2009), Biochemistry, 48, 1445-1453.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21-RIL cells | Mycolicibacterium smegmatis |
| expressed in Escherichia coli BL21-RIL cells | Pseudomonas fluorescens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complex with Mg2+, hanging drop vapor diffusion method, using 10% (v/v) 2-propanol, 0.1 M MES, pH 6.0, and 0.2 M Ca(OAc)2, or in complex with Mg2+ and cis,cis-muconate, hanging drop vapor diffusion method, using 1.0 M (NH4)2SO4, 0.1 M HEPES, pH 7.0, and 0.5% (w/v) PEG 8000 | Mycolicibacterium smegmatis |
| in complex with Mg2+, hanging drop vapor diffusion method, using 20% (v/v) PEG 1000, 0.1 M cacodylate, pH 6.5, and 0.2 M MgCl2, or in complex with Mg2+ and cis,cis-muconate, hanging drop vapor diffusion method, using 2.0 M (NH4)2SO4, 0.1 M MES, pH 6.0, and 5% (v/v) isopropanol | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
- |
- |
| Pseudomonas fluorescens | Q4K9X1 | - |
- |
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel-affinity column chromatography and gel filtration | Mycolicibacterium smegmatis |
| nickel-affinity column chromatography and gel filtration | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis,cis-muconate | - |
Pseudomonas putida | (4S)-muconolactone | - |
? |  |
| cis,cis-muconate | syn-cycloisomerization | Pseudomonas fluorescens | (4S)-muconolactone | - |
? | |
| cis,cis-muconate | syn-cycloisomerization | Mycolicibacterium smegmatis | (+)-(4S)-muconolactone | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cis,cis-muconate lactonizing enzyme | - |
Mycolicibacterium smegmatis |
| cis,cis-muconate lactonizing enzyme | - |
Pseudomonas fluorescens |
| MLE | - |
Pseudomonas putida |
| MLE-1 | - |
Pseudomonas fluorescens |
| MLE-2 | - |
Mycolicibacterium smegmatis |
| Muconate lactonizing enzyme | - |
Mycolicibacterium smegmatis |
| Muconate lactonizing enzyme | - |
Pseudomonas putida |
| Muconate lactonizing enzyme | - |
Pseudomonas fluorescens |
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Release 2023.1 (January 2023)
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