Crystallization (Comment) | Organism |
---|---|
sitting-drop vapor diffusion method | Pseudomonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
T52G | turnover number with cis,cis-muconate is 52.5fold lower than that of the wild-type enzyme, Km-value for cis,cis-muconate is 3.9fold higher than that of wild-type enzyme | Pseudomonas sp. |
T52G/F103S | turnover number with cis,cis-muconate is 12fold lower than that of the wild-type enzyme, Km-value for cis,cis-muconate is 3.6fold higher than that of wild-type enzyme | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
cis,cis-muconate | wild-type enzyme | Pseudomonas sp. | |
0.143 | - |
cis,cis-muconate | mutant enzyme T52G/T103S | Pseudomonas sp. | |
0.157 | - |
cis,cis-muconate | mutant enzyme T52G | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | active enzyme contains Mn2+ | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis,cis-muconate | Pseudomonas sp. | enzyme is involved in beta-ketoadipate pathway | muconolactone | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
P51 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis,cis-muconate | - |
Pseudomonas sp. | muconolactone | - |
? | |
cis,cis-muconate | enzyme is involved in beta-ketoadipate pathway | Pseudomonas sp. | muconolactone | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
cis,cis-muconate | mutant enzyme T52G | Pseudomonas sp. | |
17.5 | - |
cis,cis-muconate | mutant enzyme T52G/F103S | Pseudomonas sp. | |
210 | - |
cis,cis-muconate | wild-type enzyme | Pseudomonas sp. |