Literature summary for 5.4.99.B22 extracted from

Nakamoto, M.A.; Lovejoy, A.F.; Cygan, A.M.; Boothroyd, J.C.
mRNA pseudouridylation affects RNA metabolism in the parasite Toxoplasma gondii (2017), RNA, 23, 1834-1849 .

Search for more literature for 5.4.99.B22

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Toxoplasma gondii	extensive, regulated pseudouridylation of Toxoplasma mRNA with evidence for 1669 and 394 sites of pseudouridylation in tachyzoite and bradyzoite mRNAs, respectively. The distribution of Us and Psis is significantly different between the three regions of the transcript. Pseudouridylation is underrepresented in the 3'-UTR. At least in tachyzoites, TgPUS1-dependent Psis are distributed within the codon comparably toTgPUS1-independent Psis	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Toxoplasma gondii	B6V6I8	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
bradyzoite	-	Toxoplasma gondii	-
tachyzoite	-	Toxoplasma gondii	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	extensive, regulated pseudouridylation of Toxoplasma mRNA with evidence for 1669 and 394 sites of pseudouridylation in tachyzoite and bradyzoite mRNAs, respectively. The distribution of Us and Psis is significantly different between the three regions of the transcript. Pseudouridylation is underrepresented in the 3'-UTR. At least in tachyzoites, TgPUS1-dependent Psis are distributed within the codon comparably toTgPUS1-independent Psis	Toxoplasma gondii	?	-	?
additional information	identification of TgPUS1-dependent Psis in Toxoplasma RNA. Psis are relatively depleted in the 3'-UTR but enriched at position 1 of codons, identification by CMCT treatment combined with deep sequencing. Human rRNA data can be used to establish criteria for identification of Psis with high confidence, method evaluation, overview. PSI-seq identifies evolutionarily conserved sites of pseudouridylation in Toxoplasma gondii rRNAs. Determination of three TgPUS1-dependent Psis, all in the 5' half of the anticodon arm, specifically at positions 27 and 34 of tRNA Val and Asn (TGME49_251700 and TGME49_293750) in tachyzoites and position 28 of tRNA Ile (TGME49_262568) in bradyzoites	Toxoplasma gondii	?	-	?

Synonyms

Synonyms	Comment	Organism
TgPUS1	-	Toxoplasma gondii

General Information

General Information	Comment	Organism
malfunction	PUS1 gene disruption mutants of Toxoplasma gondii are defective in differentiation from tachyzoites to bradyzoites. Pseudouridylation of the 5'-UTR and coding regions has a modest effect on steady-state mRNA levels, and the mRNA stability is modestly affected by TgPUS1-dependent pseudouridylation	Toxoplasma gondii
additional information	pseudouridines in Toxoplasma snRNAs are homologous to those in Saccharomyces cerevisiae and human snRNAs, and are not TgPUS1-dependent	Toxoplasma gondii
physiological function	a pseudouridine synthase (TgPUS1) is necessary for differentiation of the single celled eukaryotic parasite Toxoplasma gondii from active to chronic infection. Many Psis in tRNA and mRNA are dependent on the action of TgPUS1, and TgPUS1-dependent mRNA Psis are enriched in developmentally regulated transcripts. Genes containing a TgPUS1-dependent Psi are relatively more abundant in mutant parasites, mRNAs containing TgPUS1-dependent Psi have a modest but statistically significant increase in half-life in the mutant parasites. mRNA Psis play an important biological role. PUS is necessary for differentiation of the parasite Toxoplasma gondii. Pseudouridylation of spliceosomal RNAs is TgPUS1-independent	Toxoplasma gondii

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Release 2023.1 (January 2023)