Cloned (Comment) | Organism |
---|---|
transcriptome-wide quantitative mapping of Psi | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | for tRNA and Pus7, many known sites and their associated modifiers are identified, including Pus1-mediated Psi sites at positions 26-28 in several tRNAs, Pus7-dependent sites at position 13 of glutamate tRNA, Pus9-dependent site in mitochondrial aspartate tRNA at position 32, and Deg1-dependent sites at positions 37-38 in several tRNAs. Pseudouridylation in ncRNAs at sites of inter or intra-molecular interactions | ? | - |
? | |
tRNA uridine13 | Saccharomyces cerevisiae | - |
tRNA pseudouridine13 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q08647 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | for tRNA and Pus7, many known sites and their associated modifiers are identified, including Pus1-mediated Psi sites at positions 26-28 in several tRNAs, Pus7-dependent sites at position 13 of glutamate tRNA, Pus9-dependent site in mitochondrial aspartate tRNA at position 32, and Deg1-dependent sites at positions 37-38 in several tRNAs. Pseudouridylation in ncRNAs at sites of inter or intra-molecular interactions | Saccharomyces cerevisiae | ? | - |
? | |
tRNA uridine13 | - |
Saccharomyces cerevisiae | tRNA pseudouridine13 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PUS7 | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
additional information | transcriptome-wide quantitative mapping of Psi, method development using Psi-seq, relying on the unique stability of N3-[N-cyclohexyl-N'-beta-(4-methylmorpholinium)ethylcarbodiimide-Psi] (N3-CMC-Psi) to alkaline hydrolysis, and the ability of N3-CMC-Psi to terminate reverse transcription. For rRNA, 24/24 Cbf5-dependent sites are known targets of pseudouridylation, 8/9 associations between snoRNAs and rRNA sites are known, as is the identified Pus5-mediated Psi site in mitochondrial 21S rRNA. One snR3-dependent site at position 2140 on 25S rRNA is not specificated, detailed overview. Cbf5-dependent Psi sites in mRNAs and snoRNAs are likely snoRNA-guided | Saccharomyces cerevisiae |
physiological function | the most abundant RNA modification is pseudouridine (Psi), Psi is ubiquitous in diverse RNAs, and dynamic in mRNA. Pseudouridylation profiles and mechanisms. Pus7-dependent induction of pseudouridylation during heat shock | Saccharomyces cerevisiae |