Cloned (Comment) | Organism |
---|---|
recombinant Pus1, tagged with six histidine residues at its N-terminus is expressed in Escherichia coli | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | the complex Pus1/tRNAVal is sensitive to the presence of EDTA | Saccharomyces cerevisiae | |
KCl | the complex of Pus1 with tRNA is sensitive to high ionic strength. The optimal concentration of KCl for Pus1 binding is 100 mM. Above 400 mM KCl, no complex is detected | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00042 | - |
tRNAVal uridine27 | pH and temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00074 | - |
tRNAIle uridine27 | pH and temperature not specified in the publication | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | the complex of Pus1 with tRNA is sensitive to high ionic strength. The optimal concentration of KCl for Pus1 binding is 100 mM. Above 400 mM KCl, no complex is detected | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q12211 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pus1 from Saccharomyces cerevisiae is a multisite-specific enzyme that catalyses the formation of pseudouridine residues at different positions in several tRNA transcripts | Saccharomyces cerevisiae | ? | - |
? | |
tRNAIle uridine27 | - |
Saccharomyces cerevisiae | tRNAIle pseudouridine27 | - |
? | |
tRNAVal uridine27 | - |
Saccharomyces cerevisiae | tRNAVal pseudouridine27 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PUS1 | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
tRNAVal uridine27 | pH and temperature not specified in the publication | Saccharomyces cerevisiae | |
0.0083 | - |
tRNAIle uridine27 | pH and temperature not specified in the publication | Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.26 | - |
tRNAIle uridine27 | pH and temperature not specified in the publication | Saccharomyces cerevisiae | |
15.95 | - |
tRNAVal uridine27 | pH and temperature not specified in the publication | Saccharomyces cerevisiae |