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Literature summary for 5.4.99.B22 extracted from

  • Arluison, V.; Buckle, M.; Grosjean, H.
    Pseudouridine synthetase Pus1 of Saccharomyces cerevisiae: kinetic characterisation, tRNA structural requirement and real-time analysis of its complex with tRNA (1999), J. Mol. Biol., 289, 491-502.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant Pus1, tagged with six histidine residues at its N-terminus is expressed in Escherichia coli Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
EDTA the complex Pus1/tRNAVal is sensitive to the presence of EDTA Saccharomyces cerevisiae
KCl the complex of Pus1 with tRNA is sensitive to high ionic strength. The optimal concentration of KCl for Pus1 binding is 100 mM. Above 400 mM KCl, no complex is detected Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00042
-
tRNAVal uridine27 pH and temperature not specified in the publication Saccharomyces cerevisiae
0.00074
-
tRNAIle uridine27 pH and temperature not specified in the publication Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
KCl the complex of Pus1 with tRNA is sensitive to high ionic strength. The optimal concentration of KCl for Pus1 binding is 100 mM. Above 400 mM KCl, no complex is detected Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q12211
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Pus1 from Saccharomyces cerevisiae is a multisite-specific enzyme that catalyses the formation of pseudouridine residues at different positions in several tRNA transcripts Saccharomyces cerevisiae ?
-
?
tRNAIle uridine27
-
Saccharomyces cerevisiae tRNAIle pseudouridine27
-
?
tRNAVal uridine27
-
Saccharomyces cerevisiae tRNAVal pseudouridine27
-
?

Synonyms

Synonyms Comment Organism
PUS1
-
Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0067
-
tRNAVal uridine27 pH and temperature not specified in the publication Saccharomyces cerevisiae
0.0083
-
tRNAIle uridine27 pH and temperature not specified in the publication Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11.26
-
tRNAIle uridine27 pH and temperature not specified in the publication Saccharomyces cerevisiae
15.95
-
tRNAVal uridine27 pH and temperature not specified in the publication Saccharomyces cerevisiae