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Literature summary for 5.4.99.8 extracted from

  • Beastall, G.H.; Rees, H.H.; Goodwin, T.W.
    Properties of a 2,3-oxidosqualene-cycloartenol cyclase from Ochromonas malhamensis (1971), FEBS Lett., 18, 175-178.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2,3-iminosqualene
-
Ochromonas malhamensis
dithiothreitol stimulates Ochromonas malhamensis
sodium deoxycholate inhibition at levels greater than 0.5%, stimulates at low concentrations, optimal activation at 0.1% Ochromonas malhamensis

Inhibitors

Inhibitors Comment Organism Structure
KCl stimulation at concentrations up to 0.35 M, at higher levels slight fall of activity Ochromonas malhamensis
p-hydroxymercuribenzoate
-
Ochromonas malhamensis
sodium deoxycholate stimulates at low concentrations, optimal activation at 0.1%. Inhibition at levels greater than 0.5% Ochromonas malhamensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
(S)-2,3-epoxysqualene
-
Ochromonas malhamensis

Metals/Ions

Metals/Ions Comment Organism Structure
KCl enzyme is inactive in absence of KCl, stimulation at concentrations up to 0.35 M, at higher levels slight fall of activity Ochromonas malhamensis

Organism

Organism UniProt Comment Textmining
Ochromonas malhamensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Ochromonas malhamensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00019
-
-
Ochromonas malhamensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2,3-Epoxysqualene
-
Ochromonas malhamensis Cycloartenol
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
30 min incubation Ochromonas malhamensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.3
-
Ochromonas malhamensis