Any feedback?
Literature summary for 5.4.99.7 extracted from
- Lanosterol biosynthesis: the critical role of the methyl-29 group of 2,3-oxidosqualene for the correct folding of this substrate and for the construction of the five-membered D ring (2012), Chemistry, 18, 13108-13116.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 27,28-bisnoroxidosqualene | irreversible inhibition | Sus scrofa |  |
| 27-norsqualene | - |
Sus scrofa | |
| 28-noroxidosqualene | - |
Sus scrofa | |
| 29-norsqualene | - |
Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.075 | - |
(3S)-2,3-epoxy-2,3-dihydrosqualene | pH 7.4, 37°C, liver microsomes | Sus scrofa | |
| 0.231 | - |
3-[(3E,7E,15E)-16-ethyl-3,7,12,20-tetramethylhenicosa-3,7,11,15,19-pentaen-1-yl]-2,2-dimethyloxirane | pH 7.4, 37°C, liver microsomes | Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Sus scrofa | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3S)-2,3-epoxy-2,3-dihydrosqualene | Sus scrofa | - |
lanosterol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| liver microsome preparation | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Sus scrofa | lanosterol | - |
? | |
| (3S)-2,3-epoxy-2,3-dihydrosqualene | the enzyme performs the polycyclization reaction by folding the substrate in a chair-boat-chair-chair conformation | Sus scrofa | lanosterol | - |
? | |
| 27-norsqualene | - |
Sus scrofa | 30-norlanosterol + 26-normalabaricatriene | - |
? | |
| 29-norsqualene | - |
Sus scrofa | (2S,4aS,6aR,7R,10aR,12aR)-1,1,4a,6a,10a-pentamethyl-7-(4-methylpent-3-en-1-yl)-1,2,3,4,4a,5,6,6a,7,8,9,10,10a,11,12,12a-hexadecahydrochrysen-2-ol + (2S,4aS,6aS,7R,10aS,12aR)-1,1,4a,6a,10a-pentamethyl-7-(4-methylpent-3-en-1-yl)-1,2,3,4,4a,5,6,6a,7,8,9,10,10a,11,12,12a-hexadecahydrochrysen-2-ol | - |
? | |
| 3-[(3E,7E,15E)-16-ethyl-3,7,12,20-tetramethylhenicosa-3,7,11,15,19-pentaen-1-yl]-2,2-dimethyloxirane | ethylation at C10 or C15 | Sus scrofa | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Sus scrofa |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000013 | - |
27-norsqualene | pH 7.4, 37°C, liver microsomes | Sus scrofa | |
| 0.000015 | - |
29-norsqualene | pH 7.4, 37°C, liver microsomes | Sus scrofa | |
| 0.00002 | - |
28-noroxidosqualene | pH 7.4, 37°C, liver microsomes | Sus scrofa | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.076 | - |
pH 7.4, 37°C, liver microsomes | Sus scrofa | 29-norsqualene | |
| 0.18 | - |
pH 7.4, 37°C, liver microsomes | Sus scrofa | 27-norsqualene | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
