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Literature summary for 5.4.99.7 extracted from

  • Wu, T.K.; Chang, Y.C.; Liu, Y.T.; Chang, C.H.; Wen, H.Y.; Li, W.H.; Shie, W.S.
    Mutation of isoleucine 705 of the oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae affects lanosterols C/D-ring cyclization and 17alpha/beta-exocyclic side chain stereochemistry (2011), Org. Biomol. Chem., 9, 1092-1097.
    View publication on PubMed
Search for more literature for 5.4.99.7

Protein Variants

Protein Variants Comment Organism
F699C the mutant is inactive Saccharomyces cerevisiae
F699C/I705F the mutant is inactive Saccharomyces cerevisiae
F699M the mutant produces 1% lanosterol, 17% (13alphaH)-isomalabarica-14E,17E,21-dien-3beta-ol, 18% (13alphaH)-isomalabarica-14Z,17E,21-dien-3beta-ol, 46% protosta-13(17),24-dien-3beta-ol, 1% 17alpha-protosta-20,24-dien-3beta-ol, 10% protosta-17(20),24-dien-3beta-ol and 7% malabarica-14E,17E,21-trien-3beta-ol from (3S)-2,3-oxidosqualene Saccharomyces cerevisiae
F699M/I705F the mutant produces 53% lanosterol, 17% (13alphaH)-isomalabarica-14E,17E,21-dien-3beta-ol, 12% (13alphaH)-isomalabarica-14Z,17E,21-dien-3beta-ol, 15% protosta-13(17),24-dien-3beta-ol, and 3% 17alpha-protosta-20(22),24-dien-3beta-ol from (3S)-2,3-oxidosqualene Saccharomyces cerevisiae
F699T the mutant produces less than 0.2% lanosterol and more than 99.8% protosta-13(17),24-dien-3beta-ol from (3S)-2,3-oxidosqualene Saccharomyces cerevisiae
F699T/I105F the mutant produces 36% lanosterol, 45% protosta-13(17),24-dien-3beta-ol, 7% 17alpha-protosta-20,24-dien-3beta-ol, and 12% 17alpha-protosta-20(22),24-dien-3beta-ol from (3S)-2,3-oxidosqualene Saccharomyces cerevisiae
I705A the mutant produces 78% lanosterol, 13% (13alphaH)-isomalabarica-14E,17E,21-dien-3beta-ol, 8% (13alphaH)-isomalabarica-14Z,17E,21-dien-3beta-ol, 0.3% protosta-13(17),24-dien-3beta-ol, and 0.7% protosta-16,24-dien-3beta-ol from (3S)-2,3-oxidosqualene Saccharomyces cerevisiae
I705C the mutant produces 72% lanosterol, 16% (13alphaH)-isomalabarica-14E,17E,21-dien-3beta-ol, 11.7% (13alphaH)-isomalabarica-14Z,17E,21-dien-3beta-ol, and 0.3% protosta-13(17),24-dien-3beta-ol from (3S)-2,3-oxidosqualene Saccharomyces cerevisiae
I705D the mutant produces 37% lanosterol, 32% (13alphaH)-isomalabarica-14E,17E,21-dien-3beta-ol, 30% (13alphaH)-isomalabarica-14Z,17E,21-dien-3beta-ol, and 1% protosta-13(17),24-dien-3beta-ol from (3S)-2,3-oxidosqualene Saccharomyces cerevisiae
I705F the mutant produces 25% lanosterol, 21(13alphaH)-isomalabarica-14(26),17E,21-trien-3beta-ol, 6% 17alpha-protosta-20,24-dien-3beta-ol, 42% 17alpha-protosta-20(22),24-dien-3beta-ol from, and 6% protosta-16,24-dien-3beta-ol from (3S)-2,3-oxidosqualene Saccharomyces cerevisiae
I705G the mutant produces 35% lanosterol, 23% (13alphaH)-isomalabarica-14E,17E,21-dien-3beta-ol, 34% (13alphaH)-isomalabarica-14Z,17E,21-dien-3beta-ol, 6% protosta-13(17),24-dien-3beta-ol, 1% 17alpha-protosta-20(22),24-dien-3beta-ol and 1% protosta-16,24-dien-3beta-ol from (3S)-2,3-oxidosqualene Saccharomyces cerevisiae
I705K the mutant produces 12% lanosterol, 26.5% (13alphaH)-isomalabarica-14E,17E,21-dien-3beta-ol, 53.1% (13alphaH)-isomalabarica-14Z,17E,21-dien-3beta-ol, 7.6% protosta-13(17),24-dien-3beta-ol, and 0.8% protosta-16,24-dien-3beta-ol from (3S)-2,3-oxidosqualene Saccharomyces cerevisiae
I705L the mutant produces 100% lanosterol from (3S)-2,3-oxidosqualene (wild-type activity) Saccharomyces cerevisiae
I705M the mutant produces 88% lanosterol, 6% (13alphaH)-isomalabarica-14E,17E,21-dien-3beta-ol, and 6% (13alphaH)-isomalabarica-14Z,17E,21-dien-3beta-ol from (3S)-2,3-oxidosqualene Saccharomyces cerevisiae
I705N the mutant produces 19% lanosterol, 41% (13alphaH)-isomalabarica-14E,17E,21-dien-3beta-ol, 37% (13alphaH)-isomalabarica-14Z,17E,21-dien-3beta-ol, and 3% protosta-13(17),24-dien-3beta-ol from (3S)-2,3-oxidosqualene Saccharomyces cerevisiae
I705P the mutant produces 81.9% lanosterol, 0.1% (13alphaH)-isomalabarica-14E,17E,21-dien-3beta-ol, 15% (13alphaH)-isomalabarica-14Z,17E,21-dien-3beta-ol, and 3% protosta-16,24-dien-3beta-ol from (3S)-2,3-oxidosqualene Saccharomyces cerevisiae
I705Q the mutant produces 25% lanosterol, 39% (13alphaH)-isomalabarica-14E,17E,21-dien-3beta-ol, 31% (13alphaH)-isomalabarica-14Z,17E,21-dien-3beta-ol, and 5% protosta-13(17),24-dien-3beta-ol from (3S)-2,3-oxidosqualene Saccharomyces cerevisiae
I705S the mutant produces 12% lanosterol, 42% (13alphaH)-isomalabarica-14E,17E,21-dien-3beta-ol, 44% (13alphaH)-isomalabarica-14Z,17E,21-dien-3beta-ol, 1% protosta-13(17),24-dien-3beta-ol, and 1%protosta-16,24-dien-3beta-ol from (3S)-2,3-oxidosqualene Saccharomyces cerevisiae
I705T the mutant produces 22% lanosterol, 36% (13alphaH)-isomalabarica-14E,17E,21-dien-3beta-ol, 41% (13alphaH)-isomalabarica-14Z,17E,21-dien-3beta-ol, and 1% protosta-13(17),24-dien-3beta-ol from (3S)-2,3-oxidosqualene Saccharomyces cerevisiae
I705V the mutant produces 75% lanosterol, 10% (13alphaH)-isomalabarica-14E,17E,21-dien-3beta-ol, and 15% (13alphaH)-isomalabarica-14Z,17E,21-dien-3beta-ol from (3S)-2,3-oxidosqualene Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-squalene-2,3-epoxide
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 Saccharomyces cerevisiae Lanosterol
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 ?

Synonyms

Synonyms Comment Organism
ERG7
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 Saccharomyces cerevisiae
OSC
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 Saccharomyces cerevisiae
Oxidosqualene-lanosterol cyclase
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 Saccharomyces cerevisiae