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Literature summary for 5.4.99.7 extracted from

  • Wu, T.K.; Wang, T.T.; Chang, C.H.; Liu, Y.T.; Shie, W.S.
    Importance of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase tyrosine 707 residue for chair-boat bicyclic ring formation and deprotonation reactions (2008), Org. Lett., 10, 4959-4962.
    View publication on PubMed
Search for more literature for 5.4.99.7

Cloned(Commentary)

Cloned (Comment) Organism
mutants are expressed in Saccharomyces cerevisiae strain TKW14 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
Y707A the mutant produces 18.9% (9R,10S)-polypoda-8(26),13E,17E,21-tetraen-3beta-ol, 56.9% lanosterol, 19.9% 9beta-lanosta-7,24-dien-3beta-ol, and 4.3% parkeol from (S)-2,3-oxidosqualene Saccharomyces cerevisiae
Y707C the mutant produces 6% (9R,10S)-polypoda-8(26),13E,17E,21-tetraen-3beta-ol, 47.1% lanosterol, 42.6% 9beta-lanosta-7,24-dien-3beta-ol, and 4.3% parkeol from (S)-2,3-oxidosqualene Saccharomyces cerevisiae
Y707D the mutant produces 21.8% (9R,10S)-polypoda-8(26),13E,17E,21-tetraen-3beta-ol, 67.3% lanosterol, 7.6% 9beta-lanosta-7,24-dien-3beta-ol, and 3.3% parkeol from (S)-2,3-oxidosqualene Saccharomyces cerevisiae
Y707E the mutant produces 12.3% (9R,10S)-polypoda-8(26),13E,17E,21-tetraen-3beta-ol, 70.8% lanosterol, 10.5% 9beta-lanosta-7,24-dien-3beta-ol, and 6.4% parkeol from (S)-2,3-oxidosqualene Saccharomyces cerevisiae
Y707F the mutant produces 89.2% lanosterol, 3.1% parkeol, and 7.7% 9beta-lanosta-7,24-dien-3beta-ol from (S)-2,3-oxidosqualene Saccharomyces cerevisiae
Y707G the mutant produces 28.3% (9R,10S)-polypoda-8(26),13E,17E,21-tetraen-3beta-ol, 48.2% lanosterol, 16.3% 9beta-lanosta-7,24-dien-3beta-ol, and 7.2% parkeol from (S)-2,3-oxidosqualene Saccharomyces cerevisiae
Y707H the mutant produces 83.6% (9R,10S)-polypoda-8(26),13E,17E,21-tetraen-3beta-ol, 4.7% lanosterol, 6.2% 9beta-lanosta-7,24-dien-3beta-ol, and 5.5% parkeol from (S)-2,3-oxidosqualene Saccharomyces cerevisiae
Y707I the mutant produces 87.9% lanosterol and 12.1% 9beta-lanosta-7,24-dien-3beta-ol from (S)-2,3-oxidosqualene Saccharomyces cerevisiae
Y707K the mutant produces 100% lanosterol from (S)-2,3-oxidosqualene (wild type reaction) Saccharomyces cerevisiae
Y707L the mutant produces 85.3% lanosterol and 14.7% 9beta-lanosta-7,24-dien-3beta-ol from (S)-2,3-oxidosqualene Saccharomyces cerevisiae
Y707M the mutant produces 100% lanosterol from (S)-2,3-oxidosqualene (wild type reaction) Saccharomyces cerevisiae
Y707N the mutant produces 100% lanosterol from (S)-2,3-oxidosqualene (wild type reaction) Saccharomyces cerevisiae
Y707P the mutant produces 100% lanosterol from (S)-2,3-oxidosqualene (wild type reaction) Saccharomyces cerevisiae
Y707Q the mutant produces 82% (9R,10S)-polypoda-8(26),13E,17E,21-tetraen-3beta-ol, 4.3% lanosterol, and 13.7% parkeol from (S)-2,3-oxidosqualene Saccharomyces cerevisiae
Y707R inactive Saccharomyces cerevisiae
Y707S the mutant produces 21.3% (9R,10S)-polypoda-8(26),13E,17E,21-tetraen-3beta-ol, 42.9% lanosterol, 20.5% 9beta-lanosta-7,24-dien-3beta-ol, and 15.3% parkeol from (S)-2,3-oxidosqualene Saccharomyces cerevisiae
Y707T the mutant produces 9.8% (9R,10S)-polypoda-8(26),13E,17E,21-tetraen-3beta-ol, 65.1% lanosterol, 13.5% 9beta-lanosta-7,24-dien-3beta-ol, and 11.6% parkeol from (S)-2,3-oxidosqualene Saccharomyces cerevisiae
Y707V the mutant produces 100% lanosterol from (S)-2,3-oxidosqualene (wild type reaction) Saccharomyces cerevisiae
Y707W the mutant produces 100% lanosterol from (S)-2,3-oxidosqualene (wild type reaction) Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2,3-oxidosqualene
-
 Saccharomyces cerevisiae lanosterol
-
 ?

Synonyms

Synonyms Comment Organism
ERG7
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 Saccharomyces cerevisiae
OSC
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 Saccharomyces cerevisiae
Oxidosqualene-lanosterol cyclase
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 Saccharomyces cerevisiae