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Literature summary for 5.4.99.5 extracted from
- Characterization of the secreted chorismate mutase from the pathogen Mycobacterium tuberculosis (2005), FEBS J., 272, 375-389.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid | competitive inhibition | Mycobacterium tuberculosis |  |
| adamantane-1-phosphonate | no inhibitory effect up to concentrations of 0.1 and 1 mM | Mycobacterium tuberculosis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
chorismate | leaderless MtCM with C-terminal His tag, at 30°C and pH 7.5 | Mycobacterium tuberculosis | |
| 0.18 | - |
chorismate | untagged enzyme, at 30°C and pH 7.5 | Mycobacterium tuberculosis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | leaderless protein | Mycobacterium tuberculosis | 5737 | - |
| periplasmic space | full-length protein | Mycobacterium tuberculosis | 42597 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18600 | - |
monomer, calculated, 167-residue untagged lederless MtCM | Mycobacterium tuberculosis |
| 18600 | - |
monomer, mass spectrometry, 167-residue untagged lederless MtCM | Mycobacterium tuberculosis |
| 19540 | - |
monomer, calculated from the variant that lacks 33 N-terminal residues | Mycobacterium tuberculosis |
| 19540 | - |
monomer, mass spectrometry | Mycobacterium tuberculosis |
| 19670 | - |
monomer, calculated from the leaderless variant with additional start methionine | Mycobacterium tuberculosis |
| 19670 | - |
monomer, mass, spectrometry, leaderless variant with additional start methionine | Mycobacterium tuberculosis |
| 23000 | - |
monomer, SDS-PAGE, 167-residue untagged lederless MtCM | Mycobacterium tuberculosis |
| 35400 | - |
ultracentrifugation | Mycobacterium tuberculosis |
| 43400 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Chorismate | Mycobacterium tuberculosis | - |
Prephenate | - |
? | |
| Chorismate | Mycobacterium tuberculosis H37Rv | - |
Prephenate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycobacterium tuberculosis |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| thermal denaturation shows a sharp transition from the native to the denatured state, with a calculated Tm of 48°C. Upon subsequent cooling, the protein renatured easily | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Chorismate | - |
Mycobacterium tuberculosis | Prephenate | - |
? | |
| Chorismate | - |
Mycobacterium tuberculosis H37Rv | Prephenate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha2, ultracentrifugation, gel filtration | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chorismate mutase | - |
Mycobacterium tuberculosis |
| CM | - |
Mycobacterium tuberculosis |
| MtCM | - |
Mycobacterium tuberculosis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
calculated Tm: 48°C | Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 50 | - |
chorismate | untagged enzyme, at 30°C and pH 7.5 | Mycobacterium tuberculosis | |
| 56 | - |
chorismate | leaderless MtCM with C-terminal His tag, at 30°C and pH 7.5 | Mycobacterium tuberculosis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0037 | - |
8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid | - |
Mycobacterium tuberculosis | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Mycobacterium tuberculosis | calculated | - |
4.9 |
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