Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-Trp | no effect | Kitasatospora aureofaciens |
General Stability | Organism |
---|---|
chorismate mutase irreversibly immobilized by n-decylamine-substituted agarose allows repeated quantitative conversion of chorismate to prephenate | Kitasatospora aureofaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-Tyr | no inhibition | Kitasatospora aureofaciens | |
L-Tyr | no inhibition | Streptomyces venezuelae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
chorismate | - |
Kitasatospora aureofaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14500 | - |
x * 14500, at least 3 subunits not linked together by disulfide bridges, SDS-PAGE | Kitasatospora aureofaciens |
51000 | - |
sucrose density gradient centrifugation | Kitasatospora aureofaciens |
63000 | - |
gel filtration | Kitasatospora aureofaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kitasatospora aureofaciens | - |
- |
- |
Streptomyces venezuelae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Kitasatospora aureofaciens |
Renatured (Comment) | Organism |
---|---|
renaturation of heat-denatured enzyme | Kitasatospora aureofaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
300 | - |
- |
Kitasatospora aureofaciens |
Storage Stability | Organism |
---|---|
-25°C, in presence of PMSF, stable for at least 2 months | Kitasatospora aureofaciens |
4°C, in presence of PMSF, stable for at least 1 month | Kitasatospora aureofaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Chorismate | - |
Streptomyces venezuelae | Prephenate | - |
? | |
Chorismate | - |
Kitasatospora aureofaciens | Prephenate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 14500, at least 3 subunits not linked together by disulfide bridges, SDS-PAGE | Kitasatospora aureofaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
reversible heat inactivation above | Kitasatospora aureofaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Kitasatospora aureofaciens |