Literature summary for 5.4.99.41 extracted from
Kushiro, T.; Shibuya, M.; Ebizuka, Y.
Chimeric triterpene synthase. A possible model for multifunctional triterpene synthase (1999), J. Am. Chem. Soc., 121, 1208-1216.
No PubMed abstract available
Protein Variants
Protein Variants |
Comment |
Organism |
additional information |
construction of chimeric proteins using beta-amyrin synthase from Panax ginseng and lupeol synthase from Arabidopsis thaliana. Chimera with N-terminal half of beta-amyrin synthase and C-terminal half of lupeol synthase produces beta-amyrin and lupeol in ratio 3:1. Chimera with only the second quarter of the N-terminus from beta-amyrin synthase, produces beta-amyrin and lupeol in a 4:1 ratio, while another chimera created by mixed PCR produces beta-amyrin and lupeol in a 1:4 ratio |
Arabidopsis thaliana |
Organism
Organism |
UniProt |
Comment |
Textmining |
Arabidopsis thaliana |
Q9C5M3 |
- |
- |
Reaction
Reaction |
Comment |
Organism |
Reaction ID |
(3S)-2,3-epoxy-2,3-dihydrosqualene = lupeol |
the final proton abstraction takes place from either of the two gem-dimethyl groups in a 1:1 ratio |
Arabidopsis thaliana |
|