Literature summary for 5.4.99.30 extracted from

Kotani, A.; Tsuji, M.; Azama, Y.; Ishii, T.; Takeda, T.; Yamashita, T.; Shimojima, M.; Konishi, T.
Purification and characterization of UDP-arabinopyranose mutase from Chlamydomonas reinhardtii (2013), Biosci. Biotechnol. Biochem., 77, 1874-1878.

Search for more literature for 5.4.99.30

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.148	-	UDP-beta-L-arabinopyranose	pH 6, 25°C, native enzyme	Chlamydomonas reinhardtii
0.152	-	UDP-beta-L-arabinofuranose	pH 6, 25°C, native enzyme	Chlamydomonas reinhardtii
0.371	-	UDP-beta-L-arabinofuranose	pH 6, 25°C, recombinant enzyme	Chlamydomonas reinhardtii
10.3	-	UDP-beta-L-arabinopyranose	pH 6, 25°C, recombinant enzyme	Chlamydomonas reinhardtii

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Chlamydomonas reinhardtii	5829	-

Organism

Organism	UniProt	Comment	Textmining
Chlamydomonas reinhardtii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purified 94fold using hydrophobic chromatography and gel filtration	Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-beta-L-arabinofuranose	-	Chlamydomonas reinhardtii	UDP-beta-L-arabinopyranose	-	?

Synonyms

Synonyms	Comment	Organism
CrUAM	-	Chlamydomonas reinhardtii
UDP-arabinopyranose mutase	-	Chlamydomonas reinhardtii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	-	Chlamydomonas reinhardtii

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	enzymatic activity decreases to 24.5% after 60 min	Chlamydomonas reinhardtii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.93	-	UDP-beta-L-arabinopyranose	pH 6, 25°C, recombinant enzyme	Chlamydomonas reinhardtii
1.44	-	UDP-beta-L-arabinopyranose	pH 6, 25°C, native enzyme	Chlamydomonas reinhardtii
1.44	-	UDP-beta-L-arabinofuranose	pH 6, 25°C, recombinant enzyme	Chlamydomonas reinhardtii
5.98	-	UDP-beta-L-arabinofuranose	pH 6, 25°C, native enzyme	Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Chlamydomonas reinhardtii

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	6	-	Chlamydomonas reinhardtii

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.09	-	UDP-beta-L-arabinopyranose	pH 6, 25°C, recombinant enzyme	Chlamydomonas reinhardtii
0.7	-	UDP-beta-L-arabinofuranose	pH 6, 25°C, recombinant enzyme	Chlamydomonas reinhardtii
9.73	-	UDP-beta-L-arabinopyranose	pH 6, 25°C, native enzyme	Chlamydomonas reinhardtii
39.3	-	UDP-beta-L-arabinofuranose	pH 6, 25°C, native enzyme	Chlamydomonas reinhardtii

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Release 2023.1 (January 2023)