Literature summary for 5.4.99.30 extracted from

Konishi, T.; Miyazaki, Y.; Yamakawa, S.; Iwai, H.; Satoh, S.; Ishii, T.
Purification and biochemical characterization of recombinant rice UDP-arabinopyranose mutase generated in insect cells (2010), Biosci. Biotechnol. Biochem., 74, 191-194.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	the combination of rUAM1, rUAM2, and rUAM3 increases activity 1.7fold as compared with that of the sum total of rUAM1, rUAM2, and rUAM3. This suggests that UAM isoenzymes work synergistically to increase activity	Oryza sativa

Cloned(Commentary)

Cloned (Comment)	Organism
expression in insect cells	Oryza sativa

General Stability

General Stability	Organism
rUMA1 expressed in insect cells retains activity after repeated cycles of freezing and thawing	Oryza sativa

Inhibitors

Inhibitors	Comment	Organism	Structure
UDP	competitive inhibition, lower affinity for enzyme than substrate	Oryza sativa
UDP-Gal	activity decreases by 40% under pretreatment for 3 h	Oryza sativa
UDP-Glc	activity decreases by 40% under pretreatment for 3 h	Oryza sativa
UDP-Xyl	activity decreases by 40% under pretreatment for 3 h	Oryza sativa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0228	-	UDP-beta-L-arabinofuranose	pH 6.8, 25°C	Oryza sativa
0.0454	-	UDP-beta-L-arabinopyranose	pH 6.8, 25°C	Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
additional information	-	native mutase is likely to exist as a complex composed of several different UDP-arabinopyranose mutases	Oryza sativa
40000	-	x * 40000, SDS-PAGE	Oryza sativa
41349	-	x * 41349, calculated from sequence	Oryza sativa

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa	Q6Z4G3	-	-
Oryza sativa	Q8H8T0	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Oryza sativa

Source Tissue

Source Tissue	Comment	Organism	Textmining
seedling	-	Oryza sativa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-beta-L-arabinofuranose	the ratio of UDP-beta-L-arabinopyranose to UDP-beta-L-arabinofuranose reaches a plateau at 93:7 from both pyranose- and furanose-forming directions	Oryza sativa	UDP-beta-L-arabinopyranose	-	r

Subunits

Subunits	Comment	Organism
?	x * 40000, SDS-PAGE	Oryza sativa
?	x * 41349, calculated from sequence	Oryza sativa

Synonyms

Synonyms	Comment	Organism
UAM1	-	Oryza sativa
UAM3	-	Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Oryza sativa

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	60	30°C: about 50% of maximal activity, 60°C: about 85% of maximal activity	Oryza sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
25	-	80 h, 50% loss of activity	Oryza sativa
50	-	1 h, 50% loss of activity	Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.185	-	UDP-beta-L-arabinopyranose	pH 6.8, 25°C	Oryza sativa
0.474	-	UDP-beta-L-arabinofuranose	pH 6.8, 25°C	Oryza sativa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	pyranose forming activity	Oryza sativa
6.5	-	furanose-forming activity	Oryza sativa

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	8	pH 4.0: about 40% of maximal activity, pH 8.0: about 55% of maximal activity, pyranose forming activity	Oryza sativa
5	8	pH 5.0: about 50% of maximal activity, pH 8.0: about 50% of maximal activity, furanose-forming activity	Oryza sativa

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
1.4	-	UDP	pH 6.8, 25°C	Oryza sativa

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
20.8	-	UDP-beta-L-arabinofuranose	pH 6.8, 25°C	Oryza sativa
40.8	-	UDP-beta-L-arabinopyranose	pH 6.8, 25°C	Oryza sativa

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Release 2023.1 (January 2023)