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Literature summary for 5.4.99.27 extracted from

  • Behm-Ansmant, I.; Urban, A.; Ma, X.; Yu, Y.T.; Motorin, Y.; Branlant, C.
    The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite-multisubstrate RNA:PSI-synthase also acting on tRNAs (2003), RNA, 9, 1371-1382.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
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Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q08647
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-

Purification (Commentary)

Purification (Comment) Organism
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Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNA uridine13 Pus7p is a multisite-multisubstrate RNA:PSI-synthase. Recombinant Pus7p has tRNAAsp:pseudouridine13- and pre-RNATyr:pseudouridine35-synthase activities Saccharomyces cerevisiae tRNA pseudouridine13
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?

Synonyms

Synonyms Comment Organism
Pus7 protein
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Saccharomyces cerevisiae
RNA:pseudouridine-synthase Pus7p
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Saccharomyces cerevisiae
RNA:PSI-synthase Pus7p
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Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
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assay at Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction disruption of the PUS7 gene abolishes pseudouridine13 formation in tRNAs in vivo. Absence of pseudouridine13 formation in tRNAAsp and tRNAGlu extracted from the DELTAPUS7 strain Saccharomyces cerevisiae