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Literature summary for 5.4.99.25 extracted from

  • Gurha, P.; Joardar, A.; Chaurasia, P.; Gupta, R.
    Differential roles of archaeal box H/ACA proteins in guide RNA-dependent and independent pseudouridine formation (2007), RNA Biol., 4, 101-109.
    View publication on PubMed
Search for more literature for 5.4.99.25

Activating Compound

Activating Compound Comment Organism Structure
aGar1 protein the aCbf5 alone can produce pseudouridine55 in tRNAs that contain the canonical 3’-CCA sequence. This activity is stimulated by aGar1 Methanocaldococcus jannaschii
aNop10 protein aCbf5 and aGar1 together can function as a tRNA pseudouridine55 synthase in a guide RNA-independent manner. This activity is enhanced by aNop10, but not by L7Ae Methanocaldococcus jannaschii
protein aGar1 aCbf5 alone can also produce Y55 in tRNAs that contain the canonical 3'-CCA sequence and this activity is stimulated by aGar1 Methanocaldococcus jannaschii
protein aNOP10 aCbf5 and aGar1 together can function as a tRNA Y55 synthase in a guide RNA-independent manner. This activity is enhanced by aNop10, but not by L7Ae Methanocaldococcus jannaschii

Cloned(Commentary)

Cloned (Comment) Organism
-
 Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-

Reaction

Reaction Comment Organism Reaction ID
tRNA uridine55 = tRNA pseudouridine55 the mechanism of uridine55 recognition by Cbf5 and the bacterial TruB are different Methanocaldococcus jannaschii
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNA uridine55 aCbf5 and aGar1 together can function as a tRNA pseudouridine55 synthase in a guide RNA-independent manner. This activity is enhanced by aNop10, but not by L7Ae. The aCbf5 alone can also produce pseudouridine55 in tRNAs that contain the canonical 3’-CCA sequence and this activity is stimulated by aGar1. The presence of C (or U) and A at tRNA position 56 and 58, respectively are not essential for Cbf5-mediated PSI55 formation. Variation in the structure of the anticodon arm of the tRNA does not affect the PSI55 synthase activity Methanocaldococcus jannaschii tRNA pseudouridine55
-
 ?
tRNATrp uridine55 tRNATrp containing or lacking 3'-CCA. aCbf5 and aGar1 together can function as a tRNA Psi55 synthase in a guide RNA-independent manner. This activity is enhanced by aNop10, but not by L7Ae. The aCbf5 alone can also produce Psi55 in tRNAs that contain the canonical 3'-CCA sequence and this activity is stimulated by aGar1. tRNAs lacking 3'-CCA can be modified only by the aCbf5-aGar1 complex. The presence of conserved C (or U) and A at tRNA positions 56 and 58, respectively, is not essential for aCbf5-mediated Psi55 formation Methanocaldococcus jannaschii tRNATrp pseudouridine55
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
68
-
 assay at Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Methanocaldococcus jannaschii