Activating Compound | Comment | Organism | Structure |
---|---|---|---|
protein aNOP10 | interaction with the pseudouridine synthase aCBF5 is required for the RNA-guided RNA:pseudouridine-synthase activity. The stable anchoring of aCBF5 to tRNAs relies on its PUA domain and the tRNA CCA sequence. Nonetheless, interaction of aNOP10 with aCBF5 can counterbalance the absence of the PUA domain or the CCA sequence and more generally helps the aCBF5 tRNA:pseudouridine55-synthase activity. Substitution of the aNOP10 residue Y14 by an alanine disturbs this activity, it only impairs mildly the RNA-guided activity. The opposite effect is observed for the aNOP10 variant H31A | Pyrococcus abyssi |
Protein Variants | Comment | Organism |
---|---|---|
K53A | substitution K53A or R202A in aCBF5 impairs both the tRNA:pseudouridine55-synthase and the RNA-guided RNA:pseudouridine-synthase activities | Pyrococcus abyssi |
R202A | substitution K53A or R202A in aCBF5 impairs both the tRNA:pseudouridine55-synthase and the RNA-guided RNA:pseudouridine-synthase activities | Pyrococcus abyssi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | Q9V1A5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNA uridine55 | the stable anchoring of aCBF5 to tRNAs relies on its PUA domain and the tRNA CCA sequence | Pyrococcus abyssi | tRNA pseudouridine55 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aCBF5 | - |
Pyrococcus abyssi |
tRNA:PSI55-synthase | - |
Pyrococcus abyssi |