Localization | Comment | Organism | GeneOntology No. | Textmining |
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Organism | UniProt | Comment | Textmining |
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Escherichia coli | - |
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Synonyms | Comment | Organism |
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pseudouridine synthase RluD | - |
Escherichia coli |
RluD | - |
Escherichia coli |
General Information | Comment | Organism |
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malfunction | loss of the RluD protein and its product pseudouridine residues (pseudouridine1911, pseudouridine 1915 and pseudouridine 1917) in a deletion strain lacking the rluD gene. This strain exhibits defects in ribosome assembly, biogenesis, and function. Specifically, there is a deficit of 70S ribosomes, an increase in 50S and 30S subunits, and the appearance of new 62S (derived from the breakdown of unstable 70S ribosomes) and 39S particles (immature precursors of the 50S subunits). The defect in ribosome assembly and resulting growth phenotype of the mutant could be restored by expression of wild-type RluD and synthesis of C1911, C1915, and C1917 residues, but not by catalytically inactive mutant RluD proteins, incapable of pseudouridine formation | Escherichia coli |