Literature summary for 5.4.99.20 extracted from

Pan, H.; Ho, J.D.; Stroud, R.M.; Finer-Moore, J.
The crystal structure of E. coli rRNA pseudouridine synthase RluE (2007), J. Mol. Biol., 367, 1459-1470.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion method, crystal structures of the C-terminal, catalytic domain of Escherichia coli RluE at 1.2 A resolution and of full-length RluE at 1.6 A resolution. The crystals of the full-length enzyme contain two molecules in the asymmetric unit and in both molecules the N-terminal domain is disordered. The higher resolution, truncated RluE structure is used for analysis and for comparison to other pseudouridine synthases	Escherichia coli

Crystallization (Comment)

Organism

hanging drop vapour diffusion method, crystal structures of the C-terminal, catalytic domain of Escherichia coli RluE at 1.2 A resolution and of full-length RluE at 1.6 A resolution. The crystals of the full-length enzyme contain two molecules in the asymmetric unit and in both molecules the N-terminal domain is disordered. The higher resolution, truncated RluE structure is used for analysis and for comparison to other pseudouridine synthases

Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24900	-	24900, SDS-PAGE	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
23S rRNA uridine2457	Escherichia coli	pseudouridine synthase RluE modifies U2457 in a stem of 23S RNA in Escherichia coli. This modification is located in the peptidyl transferase center of the ribosome.The stem alone is not a good RluE substrate, suggesting RluE undergoes additional interactions with other regions in the ribosome	23S rRNA pseudouridine2457	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P75966	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
23S rRNA uridine2457	pseudouridine synthase RluE modifies U2457 in a stem of 23S RNA in Escherichia coli. This modification is located in the peptidyl transferase center of the ribosome.The stem alone is not a good RluE substrate, suggesting RluE undergoes additional interactions with other regions in the ribosome	Escherichia coli	23S rRNA pseudouridine2457	-	?
23S rRNA uridine2457	pseudouridine synthase RluE modifies U2457 in a stem of 23S RNA in Escherichia coli. This modification is located in the peptidyl transferase center of the ribosome. The stem alone is not a good RluE substrate, suggesting RluE undergoes additional interactions with other regions in the ribosome	Escherichia coli	23S rRNA pseudouridine2457	-	?

Subunits

Subunits	Comment	Organism
?	24900, SDS-PAGE	Escherichia coli

Synonyms

Synonyms	Comment	Organism
RluE	-	Escherichia coli