Cloned (Comment) | Organism |
---|---|
recombinant enzyme expression | Bacillus anthracis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-chloro-N-(5-(2,5-dichlorophenyl)-1,3,4-oxadiazol-2-yl)benzamide | 19% inhibition at 0.010 mM | Bacillus anthracis | |
4-benzoyl-N-(5-(2,4-dimethylphenyl)-1,3,4-oxadiazol-2-yl)benzamide | 13% inhibition at 0.010 mM | Bacillus anthracis | |
4-Nitro-5-aminoimidazole ribonucleotide | NAIR | Bacillus anthracis | |
Ciprofloxacin | - |
Bacillus anthracis | |
linezolid | - |
Bacillus anthracis | |
additional information | high-throughput screening of compounds binding to Bacillus anthracis PurE. A a low ionic strength buffer condition is used to accentuate the thermal shift stabilization induced by compound binding to Bacillus anthracis PurE. Computational ligand docking to the active site. Determination of inhibitory compounds for inhibition of BaPurE activity and for cytotoxicity against Bacillus anthracis (DELTAANR strain), Escherichia coli (BW25113 strain, wild-type and DELTATolC), Francisella tularensis, Staphylococcus aureus (both methicillin susceptible and methicillin-resistant strains) and Yersinia pestis. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values, overview | Bacillus anthracis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
149000 | - |
gel filtration | Bacillus anthracis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole | Bacillus anthracis | - |
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | A0A150QXP6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme to over 85% purity | Bacillus anthracis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole | - |
Bacillus anthracis | 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 17322, mass spectrometry, 8 * 17323, sequence calculation | Bacillus anthracis |
Synonyms | Comment | Organism |
---|---|---|
N5-CAIR mutase | - |
Bacillus anthracis |
N5-carboxy-amino-imidazole ribonucleotide mutase | - |
Bacillus anthracis |
PurE | - |
Bacillus anthracis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Bacillus anthracis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bacillus anthracis |