Cloned (Comment) | Organism |
---|---|
gene purE, expression of His-tagged enzyme in Escherichia coli strain BL21 Star (DE3) | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant detagged enzyme, crystallization at room temperature, 10 mg/ml protein is mixed with a reservoir solution consisting of 0.1 M sodium acetate trihydrate, pH 4.5, and 2 M ammonium sulfate, 24 h, X-ray diffraction structure determination and analysis at 1.45 Aresolution, molecular replacement using the structure of Bacillus anthracis PurE, PDB ID 1xmp, as template | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
H38F | site-directed mutagenesis of the catalytic residue renders the enzyme inactive | Staphylococcus aureus |
H38N | site-directed mutagenesis of the catalytic residue renders the enzyme inactive | Staphylococcus aureus |
H38W | site-directed mutagenesis of the catalytic residue renders the enzyme inactive | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole | Staphylococcus aureus | - |
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | A0A0H3K7Y1 | gene purE | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 Star (DE3) by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole | - |
Staphylococcus aureus | 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N5-carboxyaminoimidazole ribonucleotide synthetase | - |
Staphylococcus aureus |
PurE | - |
Staphylococcus aureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Staphylococcus aureus |
General Information | Comment | Organism |
---|---|---|
additional information | His38 is essential for function, role of His38 as a general acid/base catalyst, structure analysis, comparison to Homo sapiens class II PurE enzyme, active site structure, overview | Staphylococcus aureus |