Literature summary for 5.4.99.18 extracted from

Constantine, C.Z.; Starks, C.M.; Mill, C.P.; Ransome, A.E.; Karpowicz, S.J.; Francois, J.A.; Goodman, R.A.; Kappock, T.J.
Biochemical and structural studies of N5-carboxyaminoimidazole ribonucleotide mutase from the acidophilic bacterium Acetobacter aceti (2006), Biochemistry, 45, 8193-8208.

Search for more literature for 5.4.99.18

Cloned(Commentary)

Cloned (Comment)	Organism
enzyme is active in Escherichia coli	Acetobacter aceti

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant H59N soaked in 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, protonation of 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate C4 may occur in absence of H59	Acetobacter aceti

Protein Variants

Protein Variants	Comment	Organism
H59A	inactive	Acetobacter aceti
H59D	0.16% of wild-type activity	Acetobacter aceti
H59F	inactive	Acetobacter aceti
H59N	inactive, crystallization data	Acetobacter aceti
H59Q	almost inactive	Acetobacter aceti
H59S	inactive	Acetobacter aceti
H89D	4.4% of wild-type activity	Acetobacter aceti
H89F	14% of wild-type activity	Acetobacter aceti
H89G	91% of wild-type activity	Acetobacter aceti
H89N	2.9% of wild-type activity	Acetobacter aceti
H89V	17% of wild-type activity	Acetobacter aceti

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0007	-	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	mutant H89G, 37°C, pH 8.0	Acetobacter aceti
0.0014	-	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	mutant H89N, 37°C, pH 8.0	Acetobacter aceti
0.006	-	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	mutant H89D, 37°C, pH 8.0	Acetobacter aceti
0.008	-	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	wild-type, 37°C, pH 8.0	Acetobacter aceti
0.008	-	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	mutant H89V, 37°C, pH 8.0	Acetobacter aceti
0.009	-	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	mutant H89F, 37°C, pH 8.0	Acetobacter aceti
0.03	-	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	mutant H59Q, 37°C, pH 8.0	Acetobacter aceti
0.2	-	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	mutant H59D, 37°C, pH 8.0	Acetobacter aceti

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
18864	-	8 * 18900, calculated, 8 * 21000, SDS-PAGE, 8 * 18864, ESI-MS	Acetobacter aceti
18900	-	8 * 18900, calculated, 8 * 21000, SDS-PAGE, 8 * 18864, ESI-MS	Acetobacter aceti
21000	-	8 * 18900, calculated, 8 * 21000, SDS-PAGE, 8 * 18864, ESI-MS	Acetobacter aceti
197000	-	gel filtration	Acetobacter aceti

Organism

Organism	UniProt	Comment	Textmining
Acetobacter aceti	Q2QJL2	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	H89 is not essential and does not function as part of a proton relay system. For synthesis of 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the carboxylate moiety is positioned in a small pocket proposed to facilitate nucleotide decarboxylation. In the reverse direction, enzyme favors protonation of 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate C4	Acetobacter aceti	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
36	-	pH 8.0, 37°C, direction of decarboxylation of 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	Acetobacter aceti

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	-	Acetobacter aceti	5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole	-	r
5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole	-	Acetobacter aceti	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	-	r

Subunits

Subunits	Comment	Organism
octamer	8 * 18900, calculated, 8 * 21000, SDS-PAGE, 8 * 18864, ESI-MS	Acetobacter aceti

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
85	-	melting temperature	Acetobacter aceti

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0006	-	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	mutant H59Q, 37°C, pH 8.0	Acetobacter aceti
0.063	-	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	mutant H89N, 37°C, pH 8.0	Acetobacter aceti
0.4	-	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	mutant H89D, 37°C, pH 8.0	Acetobacter aceti
0.49	-	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	mutant H59D, 37°C, pH 8.0	Acetobacter aceti
0.97	-	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	mutant H89G, 37°C, pH 8.0	Acetobacter aceti
1.97	-	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	mutant H89F, 37°C, pH 8.0	Acetobacter aceti
2.1	-	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	mutant H89V, 37°C, pH 8.0	Acetobacter aceti
12.2	-	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate	wild-type, 37°C, pH 8.0	Acetobacter aceti

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
7	-	maximum of enzyme stability near pH 7.0	Acetobacter aceti

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Release 2023.1 (January 2023)