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Literature summary for 5.4.99.16 extracted from
- Strategy for stable and high-level expression of recombinant trehalose synthase in Escherichia coli (2012), J. Agric. Food Chem., 60, 6063-6068.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme is useful in the biotransformation process for inexpensive production of trehalose from maltose | Picrophilus torridus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression in two plasmid-bearing Escherichia coli strains BL21(DE3)/pET23a(+)-PTTS and Rosetta(DE3)/pET23a(+)-PTTS, optimized expression in Escherichia coli strains BT04-BT06 with deleted genes treA or treF or both, which encode for trehalases, overview | Picrophilus torridus |
| strategy for stable and high-level expression of recombinant trehalose synthase in Escherichia coli | Picrophilus torridus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose | Picrophilus torridus | one-step conversion via intramolecular transglucosylation reaction | alpha,alpha-trehalose | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Picrophilus torridus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose | one-step conversion via intramolecular transglucosylation reaction | Picrophilus torridus | alpha,alpha-trehalose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PTTS | - |
Picrophilus torridus |
| Trehalose synthase | - |
Picrophilus torridus |
| TSase | - |
Picrophilus torridus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Picrophilus torridus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Picrophilus torridus |
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