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Literature summary for 5.4.99.16 extracted from

  • Wu, T.; Lin, S.; Shaw, J.
    Integrated process for the purification and immobilization of recombinant trehalose synthase for trehalose production (2011), Process Biochem., 46, 1481-1485.
No PubMed abstract available
Search for more literature for 5.4.99.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta-gami B cells Picrophilus torridus

General Stability

General Stability Organism
the enzyme immobilized with highly porous cross-linked polystyrene divinylbezene-based metal chelator maintains a residual activity of ca. 80% after 24 cycles Picrophilus torridus

Organism

Organism UniProt Comment Textmining
Picrophilus torridus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.96
-
 Mn2+-loaded, immobilized enzyme at pH 6.0 and 40°C Picrophilus torridus
1.17
-
 Fe3+-loaded, immobilized enzyme at pH 6.0 and 40°C Picrophilus torridus
1.55
-
 Cu2+-loaded, immobilized enzyme at pH 6.0 and 40°C Picrophilus torridus
2.84
-
 Ni2+-loaded, immobilized enzyme at pH 6.0 and 40°C Picrophilus torridus
3.3
-
 Zn2+-loaded, immobilized enzyme at pH 6.0 and 40°C Picrophilus torridus
3.98
-
 Co2+-loaded, immobilized enzyme at pH 6.0 and 40°C Picrophilus torridus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltose
-
 Picrophilus torridus alpha,alpha-trehalose
-
 ?

Synonyms

Synonyms Comment Organism
PTTS
-
 Picrophilus torridus
Trehalose synthase
-
 Picrophilus torridus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 immobilized enzyme Picrophilus torridus
45
-
 free enzyme Picrophilus torridus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50
-
 at 50°C a higher relative activity, 88.8%, is observed for the immobilized enzyme, compared to 76.92% for the free enzyme Picrophilus torridus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 both the free and the immobilized enzyme exhibit an optimal reaction pH of 6.0 Picrophilus torridus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8 the enzyme activity declines significantly at pH above 6.5. At pH 8.0 a complete loss in enzyme activity is observed for both the free and the immobilized enzyme. At pH5.0, the immobilized enzyme exhibits a relative activity, 82.13%, higher than the free enzyme, 50.57%. Picrophilus torridus