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Literature summary for 5.4.99.16 extracted from
- Immobilization on chitosan of a thermophilic trehalose synthase from Thermus thermophilus HJ6 (2010), J. Microbiol. Biotechnol., 20, 513-517.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Thermus thermophilus |
General Stability
| General Stability | Organism |
|---|---|
| when tested in batch reaction, the immobilized enzyme retains its relative activity of 53% after 30 reuses of reaction within 12 days | Thermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
- |
- |
| Thermus thermophilus HJ6 | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 150 days, retains 82% of its initial activity | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose | - |
Thermus thermophilus | alpha,alpha-trehalose + D-glucose + maltose | the product is composed of alpha,alpha-trehalose (48%), D-glucose (20%), maltose (32%) | ? |  |
| maltose | - |
Thermus thermophilus HJ6 | alpha,alpha-trehalose + D-glucose + maltose | the product is composed of alpha,alpha-trehalose (48%), D-glucose (20%), maltose (32%) | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Trehalose synthase | - |
Thermus thermophilus |
| TSase | - |
Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 80 | both free and chitosan-immobilized enzymes | Thermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 80 | the half-life of heat inactivation for free and chitosan-immobilized enzymes is 5.7 and 6.3 days at 70°C, respectively. The free enzyme displays complete loss of activity after 8 days at 80°C, whereas the chitosan-immobilized enzyme still retains about 25% of the initial activity | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
both free and chitosan-immobilized enzymes | Thermus thermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Thermus thermophilus |
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Release 2023.1 (January 2023)
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