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Literature summary for 5.4.99.15 extracted from

  • Di Lernia, I.; Morana, A.; Ottombrino, A.; Fusco, S.; Rossi, M.; De Rosa, M.
    Enzymes from Sulfolobus shibatae for the production of trehalose and glucose from starch (1998), Extremophiles, 2, 409-416.
    View publication on PubMed
Search for more literature for 5.4.99.15

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 maltohexaose pH 5.5, 75°C Saccharolobus shibatae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 gel filtration Saccharolobus shibatae

Organism

Organism UniProt Comment Textmining
Saccharolobus shibatae
-
-
-
Saccharolobus shibatae DMS 5389
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus shibatae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
550
-
 pH 5.5, 75°C Saccharolobus shibatae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dextrin the enzyme transforms starch and dextrins to the corresponding trehalosyl derivatives with an intramolecular transglycosylation process that converts the glucosidic linkage at the reducing end from alpha-1,4 to alpha-1,1 Saccharolobus shibatae ?
-
 ?
dextrin the enzyme transforms starch and dextrins to the corresponding trehalosyl derivatives with an intramolecular transglycosylation process that converts the glucosidic linkage at the reducing end from alpha-1,4 to alpha-1,1 Saccharolobus shibatae DMS 5389 ?
-
 ?
Maltohexaose
-
 Saccharolobus shibatae ?
-
 ?
Maltohexaose
-
 Saccharolobus shibatae DMS 5389 ?
-
 ?
maltohexaose
-
 Saccharolobus shibatae trehalosylmaltotetraose
-
 ?
maltohexaose
-
 Saccharolobus shibatae DMS 5389 trehalosylmaltotetraose
-
 ?
starch the enzyme transforms starch and dextrins to the corresponding trehalosyl derivatives with an intramolecular transglycosylation process that converts the glucosidic linkage at the reducing end from alpha-1,4 to alpha-1,1 Saccharolobus shibatae ?
-
 ?
starch the enzyme transforms starch and dextrins to the corresponding trehalosyl derivatives with an intramolecular transglycosylation process that converts the glucosidic linkage at the reducing end from alpha-1,4 to alpha-1,1 Saccharolobus shibatae DMS 5389 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 80000, SDS-PAGE Saccharolobus shibatae

Synonyms

Synonyms Comment Organism
SsTDFE
-
 Saccharolobus shibatae
trehalosyl dextrin-forming enzyme
-
 Saccharolobus shibatae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
 assay at Saccharolobus shibatae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 pH 4.5, 120 min, about 10% loss of activity Saccharolobus shibatae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33
-
 maltohexaose pH 5.5, 75°C Saccharolobus shibatae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Saccharolobus shibatae
5.5
-
 assay at Saccharolobus shibatae

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 9.5 4°C, 24 h, stable Saccharolobus shibatae

pI Value

Organism Comment pI Value Maximum pI Value
Saccharolobus shibatae FPLC chromatofocusing
-
 5