Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Nostoc flagelliforme |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 54% residual activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) | Nostoc flagelliforme | |
EDTA | 31% residual activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) | Nostoc flagelliforme | |
Hg2+ | 42% residual activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) | Nostoc flagelliforme | |
Mg2+ | 66% residual activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) | Nostoc flagelliforme | |
Mn2+ | 51% residual activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) | Nostoc flagelliforme |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.87 | - |
maltohexaose | recombinant enzyme, in 50 mM phosphate-citric acid buffer (0.2 M Na3PO4, 0.1 M citric acid, pH 7.0) at 50°C | Nostoc flagelliforme |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 134% relative activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) | Nostoc flagelliforme | |
Zn2+ | 122% relative activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) | Nostoc flagelliforme |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
106600 | - |
calculated from amino acid sequence | Nostoc flagelliforme |
125000 | - |
His6-tagged enzyme, SDS-PAGE | Nostoc flagelliforme |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc flagelliforme | EF433294 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA-agarose column chromatography | Nostoc flagelliforme |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltohexaose | - |
Nostoc flagelliforme | alpha-maltotetraosyl trehalose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
maltooligosyltrehalose synthase | - |
Nostoc flagelliforme |
MTS | - |
Nostoc flagelliforme |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant enzyme | Nostoc flagelliforme |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant enzyme | Nostoc flagelliforme |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Nostoc flagelliforme |
Organism | Comment | Expression |
---|---|---|
Nostoc flagelliforme | the expression of MTS is upregulated during drought stress | up |