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Literature summary for 5.4.99.15 extracted from

  • Wu, S.; Shen, R.; Zhang, X.; Wang, Q.
    Molecular cloning and characterization of maltooligosyltrehalose synthase gene from Nostoc flagelliforme (2010), J. Microbiol. Biotechnol., 20, 579-586.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Nostoc flagelliforme

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 54% residual activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) Nostoc flagelliforme
EDTA 31% residual activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) Nostoc flagelliforme
Hg2+ 42% residual activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) Nostoc flagelliforme
Mg2+ 66% residual activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) Nostoc flagelliforme
Mn2+ 51% residual activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) Nostoc flagelliforme

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.87
-
maltohexaose recombinant enzyme, in 50 mM phosphate-citric acid buffer (0.2 M Na3PO4, 0.1 M citric acid, pH 7.0) at 50°C Nostoc flagelliforme

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 134% relative activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) Nostoc flagelliforme
Zn2+ 122% relative activity at 1 mM, at 50°C in 50 mM phosphate buffer (pH 7.0) Nostoc flagelliforme

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
106600
-
calculated from amino acid sequence Nostoc flagelliforme
125000
-
His6-tagged enzyme, SDS-PAGE Nostoc flagelliforme

Organism

Organism UniProt Comment Textmining
Nostoc flagelliforme EF433294
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA-agarose column chromatography Nostoc flagelliforme

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltohexaose
-
Nostoc flagelliforme alpha-maltotetraosyl trehalose
-
?

Synonyms

Synonyms Comment Organism
maltooligosyltrehalose synthase
-
Nostoc flagelliforme
MTS
-
Nostoc flagelliforme

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
recombinant enzyme Nostoc flagelliforme

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
recombinant enzyme Nostoc flagelliforme

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
Nostoc flagelliforme

Expression

Organism Comment Expression
Nostoc flagelliforme the expression of MTS is upregulated during drought stress up