Cloned (Comment) | Organism |
---|---|
expressed in Lactococcus lactis strain NZ9000 | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87000 | - |
SDS-PAGE | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus MT4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.4 | - |
recombinant enzyme, after 45.5fold purification, at 70°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltoheptaose | preferred substrate | Saccharolobus solfataricus | alpha-maltopentaosyl trehalose | - |
? | |
maltoheptaose | preferred substrate | Saccharolobus solfataricus MT4 | alpha-maltopentaosyl trehalose | - |
? | |
maltohexaose | preferred substrate | Saccharolobus solfataricus | alpha-maltotetraosyl trehalose | - |
? | |
maltohexaose | preferred substrate | Saccharolobus solfataricus MT4 | alpha-maltotetraosyl trehalose | - |
? | |
additional information | starch, amylose and amylopectin are no good substrates, MTS is not active on maltotriose | Saccharolobus solfataricus | ? | - |
? | |
additional information | starch, amylose and amylopectin are no good substrates, MTS is not active on maltotriose | Saccharolobus solfataricus MT4 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
maltooligosyl-trehalose synthase | - |
Saccharolobus solfataricus |
MTS | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
purified recombinant enzyme | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
MTS proves stable at 75°C for more than 24 h, with a half life of about 50 h | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
purified recombinant enzyme | Saccharolobus solfataricus |