Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.4.99.11 extracted from

  • Contesini, F.; Carvalho, P.; Grosso, C.; Sato, H.
    Single-step purification, characterization and immobilization of a sucrose isomerase from Erwinia sp (2013), Biocatal. Agricult. Biotechnol., 2, 322-327.
No PubMed abstract available
Search for more literature for 5.4.99.11

Protein Variants

Protein Variants Comment Organism
additional information enzyme immobilization by adsorption on a chromatographic resin, optimal reaction conditions for the conversion of sucrose into isomaltulose catalyzed by the immobilized sucrose isomerase is at a pH range 6.0-6.5 and 35-40°C Erwinia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE Erwinia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose Erwinia sp.
-
 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose
-
 ?

Organism

Organism UniProt Comment Textmining
Erwinia sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 17.9fold by adsorption on a chromatographic resin Erwinia sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.16
-
 crude enzyme, pH 6.3, 33°C Erwinia sp.
38.75
-
 purified native enzyme, pH 6.3, 33°C Erwinia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose
-
 Erwinia sp. 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose
-
 ?
sucrose the purified sucrose isomerase converts sucrose into 63% of isomaltulose and 30% of trehalulose at pH 6.3 and 33°C. Optimal reaction conditions for the conversion of sucrose into isomaltulose catalyzed by the immobilized sucrose isomerase is at a pH range 6.0-6.5 and 35-40°C Erwinia sp. 1-O-alpha-D-glucopyranosyl-D-fructofuranose + alpha-D-glucosylpyranosyl-1,6-D-fructofuranose
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Erwinia sp.

Synonyms

Synonyms Comment Organism
sucrose isomerase
-
 Erwinia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35
-
 Erwinia sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 purified native enzyme, stable below Erwinia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
-
 Erwinia sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 purified native enzyme, stable at Erwinia sp.