Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Enterobacter sp. FMB-1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 91% residual activity at 5 mM | Enterobacter sp. FMB-1 | |
Mg2+ | 98% residual activity at 5 mM | Enterobacter sp. FMB-1 | |
Zn2+ | 96% residual activity at 5 mM | Enterobacter sp. FMB-1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 115% relative activity at 5 mM | Enterobacter sp. FMB-1 | |
EDTA | 119% relative activity at 5 mM | Enterobacter sp. FMB-1 | |
Fe2+ | 126% relative activity at 5 mM | Enterobacter sp. FMB-1 | |
Mn2+ | 164% relative activity at 5 mM | Enterobacter sp. FMB-1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
SDS-PAGE | Enterobacter sp. FMB-1 |
70180 | - |
calculated from amino acid sequence | Enterobacter sp. FMB-1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter sp. FMB-1 | B5ABD8 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, gel filtration | Enterobacter sp. FMB-1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose | - |
Enterobacter sp. FMB-1 | isomaltulose + trehalulose | the product ratio is 78% isomaltulose and 22% trehalulose | ? |
Synonyms | Comment | Organism |
---|---|---|
isomaltulose synthase | - |
Enterobacter sp. FMB-1 |
SIase | - |
Enterobacter sp. FMB-1 |
sucrose isomerase | - |
Enterobacter sp. FMB-1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Enterobacter sp. FMB-1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | about 50% activity at 20°C, about 80% activity at 30°C, 100% activity at 40-50°C, about 20% activity at 55°C, complete loss of activity at 60-70°C | Enterobacter sp. FMB-1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | - |
Enterobacter sp. FMB-1 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | the enzymatic activity is significantly lower outside the optimal pH range of 5.0-6.0 implying that the enzyme is very sensitive to pH | Enterobacter sp. FMB-1 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Enterobacter sp. FMB-1 | calculated from amino acid sequence | - |
6 |