Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Serratia plymuthica |
Crystallization (Comment) | Organism |
---|---|
native enzyme, crystals belong to space group P212121 and diffract to 1.95 A resolution | Serratia plymuthica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32 | - |
sucrose | pH 5.5, 25°C | Serratia plymuthica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67300 | - |
x * 67300, SDS-PAGE | Serratia plymuthica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia plymuthica | - |
sucrose isomerase SmuA additionally produces alpha-D-glucopyranosyl-1,1-D-fructofuranose from sucrose | - |
Purification (Comment) | Organism |
---|---|
recombinant protein | Serratia plymuthica |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
500 | - |
pH 5.5, 25°C | Serratia plymuthica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose | - |
Serratia plymuthica | alpha-D-glucopyranosyl-1,1-D-fructofuranose | plus small amounts of glucose, fructose and eventually isomaltose as by-products | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67300, SDS-PAGE | Serratia plymuthica |