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Literature summary for 5.4.99.11 extracted from

  • Ravaud, S.; Watzlawick, H.; Haser, R.; Mattes, R.; Aghajari, N.
    Overexpression, purification, crystallization and preliminary diffraction studies of the Protaminobacter rubrum sucrose isomerase SmuA (2006), Acta Crystallogr. Sect. F, F62, 74-76.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Serratia plymuthica

Crystallization (Commentary)

Crystallization (Comment) Organism
native enzyme, crystals belong to space group P212121 and diffract to 1.95 A resolution Serratia plymuthica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
32
-
sucrose pH 5.5, 25°C Serratia plymuthica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67300
-
x * 67300, SDS-PAGE Serratia plymuthica

Organism

Organism UniProt Comment Textmining
Serratia plymuthica
-
sucrose isomerase SmuA additionally produces alpha-D-glucopyranosyl-1,1-D-fructofuranose from sucrose
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Serratia plymuthica

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
500
-
pH 5.5, 25°C Serratia plymuthica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose
-
Serratia plymuthica alpha-D-glucopyranosyl-1,1-D-fructofuranose plus small amounts of glucose, fructose and eventually isomaltose as by-products ?

Subunits

Subunits Comment Organism
? x * 67300, SDS-PAGE Serratia plymuthica