Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.4.99.11 extracted from

  • Zhang, D.; Li, X.; Zhang, L.H.
    Isomaltulose synthase from Klebsiella sp. strain LX3: gene cloning and characterization and engineering of thermostability (2002), Appl. Environ. Microbiol., 68, 2676-2682.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.99.11

Cloned(Commentary)

Cloned (Comment) Organism
-
 Klebsiella sp.

Protein Variants

Protein Variants Comment Organism
E498P temperature-optimum is 40°C compared to 35°C for the wild-type enzyme, maximal specific activity increases by 7%. Half-life at 50°C is 9.45 min compared to 1.81 min for wild-type enzyme. Mutation slightly increases the ratio of turnover number to KM-value. Percent content of monosaccharide decreases from 5.9% of the wild-type enzyme to 3.4% Klebsiella sp.
E498P/R310P temperature-optimum is 45°C compared to 35°C for the wild-type enzyme, maximal specific activity increases by 16%. Half-life is 13.61 min compared to 1.81 min for wild-type enzyme. Mutation slightly increases the ratio of turnover number to KM-value. Percent content of monosaccharide decreases from 5.9% of the wild-type enzyme to 3.3% Klebsiella sp.

Inhibitors

Inhibitors Comment Organism Structure
CuSO3 14% inhibition at 1 mM, 23% inhibition at 5 mM Klebsiella sp.
EDTA 36% inhibition at 3 mM, 70% inhibition at 15 mM Klebsiella sp.
Fe3+ 5 mM FeCl3, complete inactivation Klebsiella sp.
FeCl2 25% inhibition at 1 mM, 51% inhibition at 5 mM Klebsiella sp.
Hg2+ 1 mM HgCl2, complete inactivation Klebsiella sp.
SDS 3 mM, complete inactivation Klebsiella sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
54.6
-
 sucrose pH 6.0, 35°C Klebsiella sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ enhances activity Klebsiella sp.
Mn2+ enhances activity Klebsiella sp.

Organism

Organism UniProt Comment Textmining
Klebsiella sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose
-
 Klebsiella sp. alpha-D-glucopyranosyl-1,6-D-fructofuranose + alpha-D-glucopyranosyl-1,1-D-fructofuranose the major product is alpha-D-glucopyranosyl-1,6-D-fructofuranose, small amounts of alpha-D-glucopyranosyl-1,1-D-fructofuranose ?

Synonyms

Synonyms Comment Organism
PalI
-
 Klebsiella sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 wild-type enzyme Klebsiella sp.
40
-
 mutant enzyme E498P Klebsiella sp.
45
-
 mutant enzyme E498P/R310P Klebsiella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life of wild-type enzyme: 1.81 min, half-life of mutant enzymes E498P is 9.45 min, half-life of mutant enzyme E498P/R310P is 13.61 min Klebsiella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Klebsiella sp.