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Literature summary for 5.4.99.1 extracted from
- beta-Methylaspartate-glutamate mutase from Clostridium tetanomorphum (1985), Methods Enzymol., 113, 121-133.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | component S requires treatment with 2-mercaptoethanol to show maximal activity. Component E does not require this treatment | Clostridium tetanomorphum |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetate | component S is inhibited, component E is not inhibited | Clostridium tetanomorphum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
L-threo-3-methylaspartate | - |
Clostridium tetanomorphum | |
| 1.5 | - |
L-Glu | - |
Clostridium tetanomorphum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Glu | Clostridium tetanomorphum | initial reaction in anaerobic degradation of Glu | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium tetanomorphum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of component E and component S | Clostridium tetanomorphum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Glu | initial reaction in anaerobic degradation of Glu | Clostridium tetanomorphum | ? | - |
? | |
| L-Glu | - |
Clostridium tetanomorphum | threo-3-Methylaspartate | - |
? | |
| L-threo-3-methylaspartate | - |
Clostridium tetanomorphum | L-glutamate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Clostridium tetanomorphum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
- |
Clostridium tetanomorphum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | 38 | 27°C: about 50% of maximal activity, 38°C: maximal activity | Clostridium tetanomorphum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Clostridium tetanomorphum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Cobalamin | and at least seven analogs containing bases other than dimethylbenzimidazolyl can serve as coenzymes | Clostridium tetanomorphum | |
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Release 2023.1 (January 2023)
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