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Literature summary for 5.4.4.5 extracted from

  • Hoffmann, I.; Jerneren, F.; Garscha, U.; Oliw, E.H.
    Expression of 5,8-LDS of Aspergillus fumigatus and its dioxygenase domain. A comparison with 7,8-LDS, 10-dioxygenase, and cyclooxygenase (2010), Arch. Biochem. Biophys., 506, 216-222.
    View publication on PubMed
Search for more literature for 5.4.4.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and in insect cells Aspergillus fumigatus

Protein Variants

Protein Variants Comment Organism
C1006S the hydroperoxide isomerase activity of 5,8-LDS mutant is abolished Aspergillus fumigatus
additional information replacements of Tyr and Cys in the conserved YRWH and FXXGPHXCLG sequences abolishes 8R-dioxygenase (8-DOX) and hydroperoxide isomerase activities, respectively. Val328 of 5,8-LDS does not influence the position of oxygenation Aspergillus fumigatus
Y374F mutation in the conserved sequence YRWH results in loss of linoleate 8R-lipoxygenase activity, whereas the hydroperoxide isomerase activity is retained Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data of truncated enzymes Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(8R,9Z,12Z)-8-hydroperoxy-10,12-octadecadienoate Aspergillus fumigatus
-
 (7S,8S,9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoate
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus C1KH66
-
-

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Aspergillus fumigatus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(8R,9Z,12Z)-8-hydroperoxy-10,12-octadecadienoate
-
 Aspergillus fumigatus (7S,8S,9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoate
-
 ?
(8R,9Z,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
 Aspergillus fumigatus (5S,8R,9Z,12Z)-5,8-dihydroxyoctadeca-9,12-dienoate
-
 ?

Synonyms

Synonyms Comment Organism
5,8-LDS bifunctional enzyme Aspergillus fumigatus
5,8-linoleate diol synthase bifunctional enzyme Aspergillus fumigatus