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Literature summary for 5.4.4.2 extracted from

  • Strawn, M.A.; Marr, S.K.; Inoue, K.; Inada, N.; Zubieta, C.; Wildermuth, M.C.
    Arabidopsis isochorismate synthase functional in pathogen-induced salicylate biosynthesis exhibits properties consistent with a role in diverse stress responses (2007), J. Biol. Chem., 282, 5919-5933.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0415
-
chorismate pH 7.0, 30°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid stromal protein Arabidopsis thaliana 9536
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolutely required Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
gel filtration Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9S7H8 activity is not regulated by light-dependent changes in stromal pH value, Mg2+ or redox potential
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification precursor protein of 62000 Da is cleaved to 58000 Da upon import into plastid Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.241
-
pH 7.0, 30°C, recombinant protein Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Chorismate
-
Arabidopsis thaliana Isochorismate reaction operates near equilibrium, equilibrium constant is 0.89. Enzyme does not convert chorismate directly to salicylic acid r
isochorismate
-
Arabidopsis thaliana chorismate
-
r

Subunits

Subunits Comment Organism
monomer 1 * 59000, SDS-PAGE Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
-
Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 37 more than 90% of maximum activity within this range Arabidopsis thaliana
4 44 more than 75% of maximum activity within this range Arabidopsis thaliana
70
-
no activity Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.645
-
chorismate pH 7.0, 30°C Arabidopsis thaliana
6.08
-
chorismate pH 7.0, 30°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
Arabidopsis thaliana