Literature summary for 5.4.4.1 extracted from

He, Z.; Nadeau, L.J.; Spain, J.C.
Characterization of hydroxylaminobenzene mutase from pNBZ139 cloned from Pseudomonas pseudoalcaligenes JS45: a highly associated SDS-stable enzyme catalyzing an intramolecular transfer of hydroxy groups (2000), Eur. J. Biochem., 267, 1110-1116.

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General Stability

General Stability	Organism
mutase in crude extracts is stable in the presence of 2% SDS, the partially purified enzyme loses 50% activity in the presence of 0.1% SDS after 30 sec	Pseudomonas oleovorans

Inhibitors

Inhibitors	Comment	Organism	Structure
Triton X-100	0.5%	Pseudomonas oleovorans
Zn2+	1 mM, 70% inhibition	Pseudomonas oleovorans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.15	-	(hydroxylamino)benzene	at pH 7.0	Pseudomonas oleovorans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
additional information	-	mutase forms high-molecular-mass complexes which elute with the void-volume, mutase tends to associate even in the presence of SDS	Pseudomonas oleovorans

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas oleovorans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Hitrap-SP/Hitrap-Q, filtration/precipitation, Cu2+-chelating chromatography	Pseudomonas oleovorans

Reaction

Reaction	Comment	Organism	Reaction ID
(hydroxyamino)benzene = 2-aminophenol	intramolecular hydroxyl transfer	Pseudomonas oleovorans	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
38	-	-	Pseudomonas oleovorans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(hydroxyamino)benzene	-	Pseudomonas oleovorans	2-aminophenol	-	?

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	9.5	56% and 59% of maximal activity at pH 6.5 and pH 9.5 respectively	Pseudomonas oleovorans

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Release 2023.1 (January 2023)