Cloned (Comment) | Organism |
---|---|
gene cctam, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Chondromyces crocatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chondromyces crocatus | - |
gene cctam | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration | Chondromyces crocatus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-tyrosine = 3-amino-3-(4-hydroxyphenyl)propanoate | pH-dependent stereochemic mechanism, detailed overview. The enzyme uses a retention-of-configuration mechanism at the amino migration terminus | Chondromyces crocatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine | the enzyme isomerizes (S)-alpha-tyrosine to (R)-beta-tyrosine and is stereoselective for (R)-beta-tyrosine (85%), but also forms the (S)-beta-tyrosine enantiomer (15%) through inversion of configuration at both migration termini | Chondromyces crocatus | 3-amino-3-(4-hydroxyphenyl)propanoate | - |
r | |
[3-deutero]-(2S,3R)-alpha-tyrosine | - |
Chondromyces crocatus | [3-deutero]-(2S,3S)-4'-O,2-N-di((S)-2-methylbutanoic acid)-alpha-tyrosine methyl ester + [3-deutero]-(2S,3R)-4'-O,2-N-di((S)-2-methylbutanoic acid)-alpha-tyrosine methyl ester | - |
? | |
[3-deutero]-(2S,3S)-alpha-tyrosine | - |
Chondromyces crocatus | [3-deutero]-(2S,3S)-4'-O,2-N-di((S)-2-methylbutanoic acid)-alpha-tyrosine methyl ester + ([3-deutero]-(2S,3R)-4'-O,2-N-di((S)-2-methylbutanoic acid)-alpha-tyrosine methyl ester | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TAM | - |
Chondromyces crocatus |
tyrosine aminomutase | - |
Chondromyces crocatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
31 | - |
assay at | Chondromyces crocatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Chondromyces crocatus |
General Information | Comment | Organism |
---|---|---|
additional information | cryptic stereochemistry of the enzyme mechanism, structure-function relationship, detailed overview | Chondromyces crocatus |