Crystallization (Comment) | Organism |
---|---|
crystal structure analysis | Acetoanaerobium sticklandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
D-ornithine | pH and temperature not specified in the publication | Acetoanaerobium sticklandii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12800 | - |
2 * 12800, subunit OraS + 2 * 90000, subunit OraE, alpha2beta2 | Acetoanaerobium sticklandii |
90000 | - |
2 * 12800, subunit OraS + 2 * 90000, subunit OraE, alpha2beta2 | Acetoanaerobium sticklandii |
210000 | - |
- |
Acetoanaerobium sticklandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ornithine | Acetoanaerobium sticklandii | - |
(2R,4S)-2,4-diaminopentanoate | - |
r | |
additional information | Acetoanaerobium sticklandii | subunit OraS of the enzyme is capable of forming a complex with recombinant enzyme (KamDE) containing only E1 of lysine 5,6-aminomutase, EC 5.4.3.4, and restores its allosteric regulation by ATP | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetoanaerobium sticklandii | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-ornithine = (2R,4S)-2,4-diaminopentanoate | catalytic mechanism, overview | Acetoanaerobium sticklandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ornithine | - |
Acetoanaerobium sticklandii | (2R,4S)-2,4-diaminopentanoate | - |
r | |
D-ornithine | the enzyme is highly specific for D-ornithine as a substrate, substrate binding structure, overview | Acetoanaerobium sticklandii | (2R,4S)-2,4-diaminopentanoate | - |
r | |
additional information | subunit OraS of the enzyme is capable of forming a complex with recombinant enzyme (KamDE) containing only E1 of lysine 5,6-aminomutase, EC 5.4.3.4, and restores its allosteric regulation by ATP | Acetoanaerobium sticklandii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 12800, subunit OraS + 2 * 90000, subunit OraE, alpha2beta2 | Acetoanaerobium sticklandii |
Synonyms | Comment | Organism |
---|---|---|
4,5-OAM | - |
Acetoanaerobium sticklandii |
ornithine 4,5-aminomutase | - |
Acetoanaerobium sticklandii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5'-deoxyadenosylcobalamin | shows ability to produce highly reactive 5'-deoxyadenosyl radical in enzymatic environments | Acetoanaerobium sticklandii | |
pyridoxal 5'-phosphate | stabilizes high-energy intermediates for performing challenging 1,2-amino rearrangements between adjacent carbons, binding site structure, overview | Acetoanaerobium sticklandii |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the class III dAdoCbl-dependent isomerase family | Acetoanaerobium sticklandii |
additional information | modeling of the closed conformation of the enzyme, domain motions, overview | Acetoanaerobium sticklandii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.2 | - |
D-ornithine | pH and temperature not specified in the publication | Acetoanaerobium sticklandii |