Literature summary for 5.4.3.5 extracted from

Chen, H.P.; Wu, S.H.; Lin, Y.L.; Chen, C.M.; Tsay, S.S.
Cloning, sequencing, heterologous expression, purification, and characterization of adenosylcobalamin-dependent D-ornithine aminomutase from Clostridium sticklandii (2001), J. Biol. Chem., 276, 44744-44750.

Search for more literature for 5.4.3.5

Cloned(Commentary)

Cloned (Comment)	Organism
expression of oraA and oraE genes encoding D-ornithine aminomutase in Escherichia coli	Acetoanaerobium sticklandii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.045	-	D-ornithine	-	Acetoanaerobium sticklandii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
12800	-	alpha2beta2, 2 * 12800 + 2 * 82900, deduced from nucleotide sequence	Acetoanaerobium sticklandii
82900	-	alpha2beta2, 2 * 12800 + 2 * 82900, deduced from nucleotide sequence	Acetoanaerobium sticklandii
201000	-	gel filtration	Acetoanaerobium sticklandii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-ornithine	Acetoanaerobium sticklandii	second step in the oxidation pathway of L-ornithine	(2R,4S)-2,4-diaminopentanoate	-	r

Organism

Organism	UniProt	Comment	Textmining
Acetoanaerobium sticklandii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant refolded D-ornithine aminomutase	Acetoanaerobium sticklandii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-ornithine	-	Acetoanaerobium sticklandii	(2R,4S)-2,4-diaminopentanoate	-	r
D-ornithine	second step in the oxidation pathway of L-ornithine	Acetoanaerobium sticklandii	(2R,4S)-2,4-diaminopentanoate	-	r

Subunits

Subunits	Comment	Organism
tetramer	alpha2beta2, 2 * 12800 + 2 * 82900, deduced from nucleotide sequence	Acetoanaerobium sticklandii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
6.3	-	D-ornithine	-	Acetoanaerobium sticklandii

Cofactor

Cofactor	Comment	Organism	Structure
5'-deoxyadenosylcobalamin	Km value 0.00043	Acetoanaerobium sticklandii
pyridoxal 5'-phosphate	Km value 0.0015	Acetoanaerobium sticklandii

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)