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Literature summary for 5.4.3.2 extracted from
- A novel lysine 2,3-aminomutase encoded by the yodO gene of Bacillus subtilis: characterization and the observation of organic radical intermediates (2000), Biochem. J., 348, 539-549.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| L-cysteine | required for in vitro activity | Bacillus subtilis |  |
| pyridoxal 5'-phosphate | required for in vitro activity | Bacillus subtilis | |
| Sodium dithionite | required for in vitro activity | Bacillus subtilis | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression Escherichia coli | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8 | - |
L-lysine | - |
Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | spectroscopic evidence for a [4Fe-4S]3+ cluster | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54061 | - |
4 * 54061, electrospry ionization mass spectroscopy | Bacillus subtilis |
| 224000 | - |
gel filtration | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant lysine 2,3-aminomutase, Streptomycin, ammonium sulfate, Sephacryl S-300, DEAE-Sephacryl | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.62 | - |
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-lysine | - |
Bacillus subtilis | (3S)-3,6-diaminohexanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 54061, electrospry ionization mass spectroscopy | Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Bacillus subtilis | |
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Release 2023.1 (January 2023)
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