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Literature summary for 5.4.2.9 extracted from

  • Liu, S.; Lu, Z.; Han, Y.; Jia, Y.; Howard, A.; Dunaway-Mariano, D.; Herzberg, O.
    Conformational flexibility of PEP mutase (2004), Biochemistry, 43, 4447-4453.
    View publication on PubMed
Search for more literature for 5.4.2.9

Crystallization (Commentary)

Crystallization (Comment) Organism
of wild-type and mutant D58A, both in apo state and in complex with Mg2+ Mytilus edulis

Protein Variants

Protein Variants Comment Organism
D58A crystallization data Mytilus edulis
K120A 30fold increase in Km-value Mytilus edulis
K120R 3fold increase in Km-value Mytilus edulis
L124A 40fold increase in Km-value Mytilus edulis
N122A 25fold increase in Km-value Mytilus edulis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 3-phosphonopyruvate wild-type, pH 7.5, 25°C Mytilus edulis
0.0059
-
 3-phosphonopyruvate mutant K120R, pH 7.5, 25°C Mytilus edulis
0.038
-
 3-phosphonopyruvate mutant N122A, pH 7.5, 25°C Mytilus edulis
0.046
-
 3-phosphonopyruvate mutant K120A, pH 7.5, 25°C Mytilus edulis
0.06
-
 3-phosphonopyruvate mutant L124A, pH 7.5, 25°C Mytilus edulis

Organism

Organism UniProt Comment Textmining
Mytilus edulis P56839
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phosphonopyruvate
-
 Mytilus edulis phosphoenolpyruvate
-
 r
phosphoenolpyruvate
-
 Mytilus edulis 3-phosphonopyruvate
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.006
-
 3-phosphonopyruvate mutant N122A, pH 7.5, 25°C Mytilus edulis
0.0098
-
 3-phosphonopyruvate mutant L124A, pH 7.5, 25°C Mytilus edulis
0.32
-
 3-phosphonopyruvate mutant K120R, pH 7.5, 25°C Mytilus edulis
0.34
-
 3-phosphonopyruvate mutant K120A, pH 7.5, 25°C Mytilus edulis
34
-
 3-phosphonopyruvate wild-type, pH 7.5, 25°C Mytilus edulis