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Literature summary for 5.4.2.9 extracted from

  • Seidel, H.M.; Pompliano, D.L.; Knowles, J.R.
    Phosphonate biosynthesis: molecular cloning of the gene for phosphonolpyruvate mutase from Tetrahymena pyriformis and overexpression of the gene product in Escherichia coli (1992), Biochemistry, 31, 2598-2608.
    View publication on PubMed
Search for more literature for 5.4.2.9

Cloned(Commentary)

Cloned (Comment) Organism
-
 Tetrahymena pyriformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
 Phosphonopyruvate truncated enzyme, pH 7.5 Tetrahymena pyriformis
0.065
-
 Phosphonopyruvate pH 7.5 Tetrahymena pyriformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32688
-
 x * 32688, gene expressed in Escherichia coli, value deduced from DNA sequence Tetrahymena pyriformis

Organism

Organism UniProt Comment Textmining
Tetrahymena pyriformis
-
 overexpression in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
overexpression in Escherichia coli Tetrahymena pyriformis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
113
-
-
 Tetrahymena pyriformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate
-
 Tetrahymena pyriformis 3-phosphonopyruvate
-
 ?

Subunits

Subunits Comment Organism
? x * 32688, gene expressed in Escherichia coli, value deduced from DNA sequence Tetrahymena pyriformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
60
-
 Phosphonopyruvate pH 7.5 Tetrahymena pyriformis
71
-
 Phosphonopyruvate truncated enzyme Tetrahymena pyriformis