Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 50% inhibition at 5 mM | Acinetobacter haemolyticus | |
ATP | 20% inhibition at 5 mM | Acinetobacter haemolyticus | |
Ca2+ | 50% inhibition at 5 mM | Acinetobacter haemolyticus | |
Co2+ | 78% inhibition at 5 mM | Acinetobacter haemolyticus | |
D-fructose 6-phosphate | 38% inhibition at 5 mM | Acinetobacter haemolyticus | |
D-Glucose 1,6-bisphosphate | 90% inhibition at 5 mM | Acinetobacter haemolyticus | |
Li+ | complete inhibition at 5 mM | Acinetobacter haemolyticus | |
additional information | not inhibited by Mg2+, D-glucose 6-phosphate, and EDTA | Acinetobacter haemolyticus | |
Ni2+ | 96% inhibition at 5 mM | Acinetobacter haemolyticus | |
UDP-D-glucose | 65% inhibition at 5 mM | Acinetobacter haemolyticus | |
Zn2+ | complete inhibition at 5 mM | Acinetobacter haemolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
alpha-D-mannose 1-phosphate | at pH 7.5 and 35°C | Acinetobacter haemolyticus | |
0.028 | - |
alpha-D-glucose 1-phosphate | at pH 7.5 and 35°C | Acinetobacter haemolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter haemolyticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, ion exchange column chromatography, and Sephacryl S-100 gel filtration | Acinetobacter haemolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
45.1 | - |
cell lysate, at pH 7.5 and 35°C | Acinetobacter haemolyticus |
1548 | - |
after 34.4fold purification, at pH 7.5 and 35°C | Acinetobacter haemolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate | - |
Acinetobacter haemolyticus | D-glucose 6-phosphate | - |
? | |
alpha-D-mannose 1-phosphate | - |
Acinetobacter haemolyticus | D-mannose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Acinetobacter haemolyticus |
Synonyms | Comment | Organism |
---|---|---|
PGM/PMM | bifunctional enzyme | Acinetobacter haemolyticus |
phosphoglucomutase/phosphomannomutase | bifunctional enzyme | Acinetobacter haemolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Acinetobacter haemolyticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 50 | about 10% activity at 10°C, about 30% activity at 20°C, about 60% activity at 25°C, about 80% activity at 30°C, 100% activity at 35°C, about 35% activity at 40°C, about 10% activity at 50°C | Acinetobacter haemolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Acinetobacter haemolyticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | about 3% activity at pH 3.0-4.0, about 10% activity at pH 5.0, about 80% activity at pH 6.0, 100% activity at pH 7.5, about 30% activity at pH 8.0, about 10% activity at pH 9.0 | Acinetobacter haemolyticus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
5 | - |
at pH 7.5 and 35°C | Acinetobacter haemolyticus | ADP | |
5 | - |
at pH 7.5 and 35°C | Acinetobacter haemolyticus | Ca2+ |