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Literature summary for 5.4.2.8 extracted from
- Cloning, expression and characterization of a phosphoglucomutase/phosphomannomutase from sphingan-producing Sphingomonas sanxanigenens (2013), Biotechnol. Lett., 35, 1265-1270.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | the enzyme is involved in production of sphingans, extracellular polysaccharides used as thickeners, emulsifiers and gelling agents, its overexpression increases the sphingan production of the transformed cell | Sphingomonas sanxanigenens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pgmG, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overexpression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), cloning in Escherichia coli strain DH5alpha | Sphingomonas sanxanigenens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.68 | - |
alpha-D-mannose 1-phosphate | recombinant His-tagged enzyme, pH 7.6, temperature not specified in the publication | Sphingomonas sanxanigenens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-mannose 1-phosphate | Sphingomonas sanxanigenens | - |
D-mannose 6-phosphate | - |
? | |
| alpha-D-mannose 1-phosphate | Sphingomonas sanxanigenens NX02 | - |
D-mannose 6-phosphate | - |
? | |
| additional information | Sphingomonas sanxanigenens | bifunctional enzyme with phosphoglucomutase and phosphomannomutase, EC 5.4.2.8, activities, the catalytic efficiency is about 15fold higher for glucose 1-phosphate than for mannose 1-phosphate | ? | - |
? | |
| additional information | Sphingomonas sanxanigenens NX02 | bifunctional enzyme with phosphoglucomutase and phosphomannomutase, EC 5.4.2.8, activities, the catalytic efficiency is about 15fold higher for glucose 1-phosphate than for mannose 1-phosphate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sphingomonas sanxanigenens | M1T754 | gene pgmG | - |
| Sphingomonas sanxanigenens NX02 | M1T754 | gene pgmG | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Sphingomonas sanxanigenens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-mannose 1-phosphate | - |
Sphingomonas sanxanigenens | D-mannose 6-phosphate | - |
? | |
| alpha-D-mannose 1-phosphate | - |
Sphingomonas sanxanigenens NX02 | D-mannose 6-phosphate | - |
? | |
| additional information | bifunctional enzyme with phosphoglucomutase and phosphomannomutase, EC 5.4.2.8, activities, the catalytic efficiency is about 15fold higher for glucose 1-phosphate than for mannose 1-phosphate | Sphingomonas sanxanigenens | ? | - |
? | |
| additional information | bifunctional enzyme with phosphoglucomutase and phosphomannomutase, EC 5.4.2.8, activities, the catalytic efficiency is about 15fold higher for glucose 1-phosphate than for mannose 1-phosphate | Sphingomonas sanxanigenens NX02 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x ** 49800, about, sequence calculation | Sphingomonas sanxanigenens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PGM/PMM | - |
Sphingomonas sanxanigenens |
| PgmG | - |
Sphingomonas sanxanigenens |
| phosphoglucomutase/phosphomannomutase | - |
Sphingomonas sanxanigenens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.28 | - |
alpha-D-mannose 1-phosphate | recombinant His-tagged enzyme, pH 7.6, temperature not specified in the publication | Sphingomonas sanxanigenens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Sphingomonas sanxanigenens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in biosynthesis of sphingans, extracellular polysaccharides | Sphingomonas sanxanigenens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.35 | - |
alpha-D-mannose 1-phosphate | recombinant His-tagged enzyme, pH 7.6, temperature not specified in the publication | Sphingomonas sanxanigenens | |
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